呼吸系统肺刷落细胞端粒酶活性及临床应用  

Activity of the Defluxio Cell Telomerase in Respiratory System and Application

作　　者：魏明[1] 

机构地区：[1]郑州大学第五附属医院检验科,河南郑州450052

出　　处：《中国临床医学》2006年第3期389-390,共2页Chinese Journal of Clinical Medicine

摘　　要：目的：探讨呼吸系统肺刷落细胞端粒酶活性与该系统肿瘤发生的相关性。方法：用PRAP-PCR-ELISA和PAGE-银染色法对27例小细胞肺癌,24例非小细胞肺癌,10例不典型肺增生,20例支气管肺炎,15例支原体肺炎,13例大叶性肺炎肺刷落细胞端粒酶活性进行定量和定性分析。结果：小细胞肺癌端粒酶活性为1.10±0.63,阳性率为100%；非小细胞肺癌端粒酶活性为1.0±0.59,阳性率为79%；不典型肺增生端粒酶活性为0.68±0.49,阳性率为73%；其它疾病端粒酶活性及阳性率分别是0.19±0.14和1%。结论：端粒酶活性与肿瘤的发生有关,并可为肿瘤的早期诊断提供可靠的指标。Objective：To study the relationship between activity of the defluxio cell telomerase and the generation of tumor in respiratory system. Methods： We determined the activity of the defluxio cell telomerase from 27 patients with small cell lung carcinoma, 24 patients with non- small cell lung carcinoma; 10 patients with atypical lung hyperplasia. 20 patients with branch pneumonia, 15 patients with mycoplasmal pneumonia, and 13 patients with lobar pneumonia using PRAP-PCR-ELIS and PAGE-Ag staining. Results： The activity of the defluxio cell telomerase in small cell lung carcinoma, non- small cell lung carcinoma, and atypical lung hyperplasia were 1.10±0. 63,1.05 ±0. 59 and 0. 68±0. 49, respectively. Conclusion： The activity of the defluxio cell telomerase in respiratory system may serve as a premature tumor marker for diagnosis.

关 键 词：呼吸系统 末端转移酶 酶联免疫吸附测定 

分 类 号：R645.2[医药卫生—外科学]