Introduction of AtNHX1 into beet improved salt-tolerance of transgenic plants  

作　　者：杨爱芳 Zhao Shilan Yin Xiaoyan Gao Feng Zhang Juren 

机构地区：[1]School of Life Science, Shandong University, Jinan 250100, P.R. China

出　　处：《High Technology Letters》2006年第2期182-187,共6页高技术通讯（英文版）

摘　　要：AtNHX1 gene encoding the Na ^＋/H ^＋ antiport on the vacuole membrane of Arabidopsis was transferred into small bud tips of 1-3mm in length derived from immature inflorescence cultures of six genotypes of beet （ Beta vulgaris L. ） by the infection of Agrobacterium tumefaciens and transgenic plants with improved salt-tolerance were obtained. When transgenic plants at 5-leaf stage were potted in sand and irrigated with solutions containing a range of concentrations of NaCl （171-513mM）, they showed minor symptoms of damage from salinity and better tolerance than the controls. There were considerable discrepancies of salt-tolerance between transgenic plants originated from the same genotype and also between different genotypes. After vernalization, bolting transgenic plants were enveloped with two layers of gauzes for self-pollination. T1 seedlings tolerant to 342-427mM NaCl were obtained respectively. These results revealed that it was feasible to improve salt-tolerance of beets by the introduction of AtNHX1 gene into cultured bud cells.

关 键 词：Beta vulgaris L. transgene Na^＋/H^＋ antiport ion compartmentalization SALT-TOLERANCE 

分 类 号：Q78[生物学—分子生物学] Q945.78