尖叶匍灯藓的组织培养及显微观察  

The tissue culture and microscopic observations of Plagiomnium cuspidatum

作　　者：李晓毓[1] 吴翠珍[2] 熊源新[2] 姜业芳[2] 杨志平[2] 何林[2] 

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]贵州大学生命科学学院,贵州贵阳550025

出　　处：《山地农业生物学报》2006年第3期217-222,共6页Journal of Mountain Agriculture and Biology

摘　　要：以尖叶匍灯藓Plagiomnium cuspidatum为材料,以MS、Benencke和Konp为基本培养基,研究尖叶匍灯藓的组织培养条件,初步建立了尖叶匍灯藓的再生体系,并初步确定最适宜尖叶匍灯藓生长的基本培养基为Konp培养基。在MS、Benencke和Konp培养基的基础上分别添加蔗糖,发现蔗糖对尖叶匍灯藓的生长没有明显的促进作用。同时,在MS培养基的基础上添加植物激素6-BA和2,4-D,根据生长状况和显微观察结果,初步认为6-BA和2,4-D对尖叶匍灯藓的生长在外形上无明显影响,而在显微结构上有影响。The study established preliminarily the regeneration system of Plagfomnium cuspidatum, using MS medium, Benecke medium and Konp medium as the basic mediums and considered Konp medium is the most favorable medium for the tissue culture. Adding sucrose to the former three mediums shows that sucrose has no active effect on the tissue culture of Plagiomnium cuspidatum. Furthermore, the author used MS medium as the frame of reference, added different plant growth substance （6 -BA and 2,4 -D） to MS medium respectively, and the microscopic observation of Plagiomnium cuspidatum also shows that different plant growth substance （6 - BA and 2,4 - D） has no obvious effect on the appearance of Plagiomnium cuspidatum, but has active effect on the microstructure to some extent.

关 键 词：尖叶匍灯藓 组织培养 显微观察 显微结构 

分 类 号：Q949.352[生物学—植物学] Q944.6