高效液相色谱-紫外光度法检测尿液和牛奶中多种头孢类抗生素  

High Performance Liquid Chromatography-Ultraviolet Photometric Detection for the Simultaneous Determination of Cephalosporins in Human Urine and Bovine Milk

作　　者：蔡玉娥[1] 蔡亚岐[1] 牟世芬[1] 鲁毅强[2] 

机构地区：[1]中国科学院生态环境研究中心环境化学与生态毒理学重点实验室,北京100085 [2]北京科技大学化学系,北京100083

出　　处：《分析化学》2006年第6期745-748,共4页Chinese Journal of Analytical Chemistry

基　　金：国家重点基础研究计划(973项目)(No.2003CB415001);国家自然科学基金(No.20577058;No.20475060);中国科学院优秀博士论文基金资助

摘　　要：使用新型的亲水性较强的C16硅胶反相色谱柱,以水/磷酸缓冲液/乙腈为流动相,流速1mL/min ,在17min内分离了头孢羟氨苄、头孢克洛、头孢氨苄、头孢拉定和头孢噻吩5种头孢类抗生素.分离后的化合物在紫外检测器上检测,检测波长270 nm,线性范围0.1～40 mg/L（r≥0.9993）, 5种化合物的检出限依次为9.7、6.9、5.6、6.4和4.9 μg/L.该方法成功的应用于人尿液和牛奶中头孢类抗生素的检测,2 mg/L浓度水平的加标回收率在96.5%～105%之间.A simple method for the separation and determination of cefadroxil, cefaclor, cefaloxin, cefradine and cefalotin etc. was completed on a C16 silica stationary phase packed reversed-phase column with mobile phase of water/acetonitrile/phosphoric buffer （ 1 mL/min） in about 17 min. The compounds were detected directly by ultraviolet detector at 270 nm. The linear range was 0.1 -40 mg/L（ r≥0.9993 ） and the detection limits of cefadroxil, cefaclor, cefaloxin, cefradine and cefalotin were 9.7, 6.9, 5.6, 6.4 and 4.9 μg/L, respectively. The method was applied successfully to the detection of cephalosporins in human urine and in bovine milk with the recovery of 96.5% -105% （when added sample was 2 mg/L）.

关 键 词：头孢羟氨苄 头孢克洛 头孢氨苄 头孢拉定 头孢噻吩 头孢类抗生素 高效液相色谱 紫外检测 

分 类 号：O657.32[理学—分析化学] O657.72[理学—化学]