荧光光谱法研究培氟沙星与牛血清白蛋白结合反应特征  

Studies on the Binding Reaction Features Between Pefloxacin and Bovine Serum Albumin by Fluorescence Spectrophtometry

作　　者：颜承农[1] 童金强[1] 熊丹[1] 刘义[2] 潘祖亭[2] 

机构地区：[1]长江大学化学与环境工程学院,荆州434020 [2]武汉大学化学与分子科学学院,武汉430072

出　　处：《分析化学》2006年第6期796-800,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.30170010);湖北省自然科学基金(No.2005ABA067)资助项目

摘　　要：在不同温度下,扫描了培氟沙星(PEFL)与牛血清白蛋白(BSA)作用的荧光猝灭光谱、同步荧光光谱、三维荧光光谱和紫外可见吸收光谱,分别用Stern-Volm er方程、L ineweaver-Burk方程和热力学方程等处理实验数据,得到了结合反应的相关参数KLB、ΔHθ、ΔGθ和ΔSθ等的平均值分别为8.419×103L.mol-1、150.7 kJ.mol-1、-22.88 kJ.mol-1和561.9 J.K-1,结合位点数为1.081;证实了在实验浓度和温度范围内,PEFL与BSA可结合形成具有一定结构的复合物,荧光猝灭作用符合静态猝灭作用特征,作用力主要是疏水作用力和静电作用力;为研究PEFL的药理作用和生物学效应,以及PEFL对蛋白质构像的影响等提供了重要信息。Under different temperatures, the binding of pefloxacin（PEFL） to bovine serum albumin （BSA） was studied by the fluorescence spectroscopy, three-dimensional fluorescence spectrometry, synchronous fluorescence spectrometry and ultra-violet spectruophotometry. After analyzing and disposing the fluorescence quenching data according to Sterm-Volmer equation, Lineweaver-Burk equation and thermodynamic equation, the average value of binding constant （ KLB ： 8. 419×10^3 L/mol）, thermodynamic parameters （ △H^θ, ： 150.7 kJ/mol,△G^θ： -22.88 k J/tool and △S^θ：561.9 J/K） and amounts of binding sites（ 1. 081 ） were obtained. Experimental results proved that BSA could react with pefloxacin to form a new compound in the range of experimental temperatures and concentrations, the quenching belonged to static fluorescence quenching, action force was electrostatic interaction and hydrophobic interaction each other. These provide important information for reserching the pharmacological effects and biological effects of pefloxacin, and configuration modification of BSA after adding pefloxacin

关 键 词：培氟沙星 牛血清白蛋白 荧光猝灭光谱 三维荧光光谱 紫外吸收光谱 热力学参数 

分 类 号：R96[医药卫生—药理学]