大鼠臂旁核内一氧化氮合酶参与内脏伤害性刺激的传导  

Nitric oxide synthase in the rat parabrachial nucleus involved in the transmission of visceral noxious stimulation

作　　者：李雷[1] 肖明[1] 李雪甫[1] 丁炯[1] 

机构地区：[1]南京医科大学人体解剖学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2006年第8期649-651,F0002,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生厅基金资助(NO.Q6504)

摘　　要：目的:探讨大鼠臂旁核(PBN)内一氧化氮合酶(NOS)是否参与内脏伤害性刺激的传导过程。方法:给予大鼠2%福尔马林溶液灌胃后,用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶(NADPH-d)组织化学结合Fos蛋白免疫组织化学的方法,观察Fos阳性神经元和NADPH-d阳性神经元及阳性纤维在PBN各亚核内的分布及共存情况。结果:PBN内Fos阳性神经元与NADPH-d阳性产物的分布范围部分重叠:在臂旁外侧核背亚核内,Fos阳性神经元与NADPH-d阳性神经元相互邻接,并可见部分Fos/NADPH-d双标神经元;在臂旁外侧核外亚核内,Fos阳性神经元与NADPH-d阳性纤维终末相互混杂分布。结论:大鼠PBN内NOS可能以不同的方式参与了内脏伤害性刺激的传导过程。Objective： To investigate whether the nitric oxide synthase （NOS） in the parabrachial nucleus （PBN） took part in the transmission of visceral noxious stimulation. Methods： The detailed colocalization of nicotinamide adenine dinucleotide phosphate diaphorase （NADPH-d） and Fos, as a neuronal activity marker, in the subnuclei of the PBN was mapped using NADPH-d histochemistry combined with Fos immunohistochemistry, following 2% formalin infusion into the stomach of rats. Results： The distribution of NADPH-d products and Fos positive neurons was partly overlapped in the PBN. The NADPH-d positive neurons adjoined the Fos positive neurons in the dorsal lateral subnuclear and some Fos/NADPH-d double labeled neurons were observed. The Fos positive neurons were intermingled with the NADPH-d positive fiber terminals in the external lateral subnuclear. Conclusion： NOS may be involved into the visceral noxious simulation by different methods in the PBN.

关 键 词：臂旁核 一氧化氮合酶 FOS 内脏伤害性刺激 大鼠 

分 类 号：R587.1[医药卫生—内分泌]