溴氰菊酯对大鼠脑组织线粒体膜通透性和细胞色素C表达的影响  

Effects of deltamethrin on permeability of mitochondrial membrane and expression of cytochrmne C in brain tissue of rats

作　　者：陈丹[1] 石年[1] 黄晓薇[1] 刘琳琳[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院卫生毒理学系教育部环境与健康重点实验室,武汉430030

出　　处：《中华劳动卫生职业病杂志》2006年第6期326-329,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金(30371225)

摘　　要：目的研究溴氰菊酯(DM)对大鼠脑组织线粒体膜通透性及细胞色素C表达的影响。方法Wistar大鼠随机分为实验组(4个不同染毒时间)和对照组,每组5只。实验组按12．5 mg/kg DM一次腹腔注射,5、24、48、72 h后处死；对照组一次腹腔注射5 mg/kg的色拉油,分别提取大鼠大脑皮层和海马的线粒体,测定线粒体膜通透性、细胞色素C氧化酶,并测定大鼠大脑皮层、海马CA1、CA2、CA3、CA4区细胞色素C的表达。结果实验组与对照组相比,5、24、48、72 h组的线粒体膜通透性增大；皮层和海马CA1区、CA2区5、24、48 h组(0．57±．04、0．67±0．09、0．58±0．04)、CA4区24 h组(0．81±0．18)细胞色素C表达增强,吸光度值的差异有统计学意义(P＜0．05或P＜0．01)；CA2区72 h组、CA3区及CA4区5、48、72 h组细胞色素C表达,吸光度值的差异则无统计学意义(P＞0．05)；细胞色素C氧化酶的活力则受到抑制,差异有统计学意义(P＜0．01)。结论DM能明显增加大鼠脑组织线粒体膜通透性,促使细胞色素C表达增强。Objective To study the effects of deltamethrin（DM） on the permeability of mitocbendrial membrane and the expression of cytochrome C in brain tissue of rats. Methods Wistar rots were randomizedly ditided into five groups（including four treated groups and one control group）. In the treated groups, DM of 12.5 mg/kg was administered intraperitoneally once in rats and the rots were sacrificed 5,24,48 and 72 hours laterwhile in the control group, the salad oil of 5 mg/kg was administered intraperitoneally once. The mitochondria in brain tissue of rats were extracted to measure the membrane permeability and the activity of cytochrome C oxidase as well as the expression of cytochrome C in cortex and hippocampus. Results After the treatment the permeability of mitochondrial membrane was significantly increased in the treated groups compared with the control group. The expression of cytochrome C was increased in cortex and hippocampus CA1 and CA2 5 h,24 h and 48 h groups and CA4 24 h group（0.57 ± 0.04,0.67 ± 0.09,0.58 ± 0.04） and（0.81 ±0.18）（P〈 0.05 or P 〈 0.01） while there was no significant difference in the expression of cytochrome C in cortex and hippocampus CA2 72h group and CA3 and CA4 5 h, 48 h and 72 h groups between the treated groups and the control group（ P 〉 0.05）. The activity of cytochrome C oxidase was inhibited（ P 〈 0.01）. Conclusion Deltamethrin can significantly increase the permeability of mitochondrial membrane and the expression of cytochrome C in brain tissue of rats.

关 键 词：溴氰菊酯 线粒体 细胞色素C 细胞色素C氧化酶 

分 类 号：R114[医药卫生—卫生毒理学]