哺乳期母鼠接触溴氰菊酯对其仔鼠神经行为发育的影响  

Effects of deitamethrin on neurobehavioral development of offspring of intoxicated rats

作　　者：李涛[1] 李官慧[2] 陈亮[1] 吴又桐[1] 陈江海[1] 石年[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院卫生毒理学系教育部环境与健康重点实验室,武汉430030 [2]黄石市大冶有色金属有限公司疾病控制中心

出　　处：《中华劳动卫生职业病杂志》2006年第6期330-332,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的探讨哺乳期母鼠接触溴氰菊酯(DM)对发育期仔鼠脑一氧化氮合酶(NOS)活力及神经行为发育的影响。方法妊娠大鼠随机分为对照组和2个染毒组,每组6只。染毒剂量分别为3．35和6．70 mg/kg,每只母鼠自产后1～19 d,隔日1次,经口染毒DM；对照组予等量玉米油。观察DM对出生后5、10和21 d仔鼠脑NOS活力及30d仔鼠学习、记忆能力的影响。结果2个染毒组仔鼠哺乳存活率(81．80%、78．60%)均明显降低于对照组(96．70%),差异有统计学意义(P＜0．01)；6．70 mg/kg染毒组10、21 d仔鼠体重[(16．62±2．28)、(31．34±6．94)g]明显低于对照组[(18．81±2．01)、(36．21±7．01)g],差异有统计学意义(P＜0．05)；6．70mg/kg染毒组仔鼠延迟时间为(3．05±1．20)s,被动逃避反应阳性率为21．5%；3．35 mg/kg染毒组被动逃避反应阳性率为22．5%,与对照组比较,差异均有统计学意义(P＜0．05)。旷场实验中,2个染毒组仔鼠行进格子数均明显降低,与对照组的差异均有统计学意义(P＜0．05)；产后5至21 d仔鼠脑NOS活力呈发育性增高趋势；6．70mg/kg染毒组出生后5 d仔鼠脑NOS活力[(0．60±0．07)U·mg pro^(-1)·h^(-1)]明显低于对照组,差异有统计学意义(P＜0．05)结论哺乳期母鼠接触DM可导致仔鼠神经行为发育迟缓,并伴有NOS活力下降。Objective To investigate the effects of deltamethrin on the filial brain nitric oxide synthase （NOS） activity and neurobehavioral development of the exposed lactatianal rats. Methods Pregnant rats were randomizedly divided into the treated group and the control group.The treated group was administered orally with 3.35, 6.70 mg/kg dehamethrin every other day from postnatal day（PND） 1 to PND 19 while the control group was administered with the corn oil of same amount in the same period. The activity of NOS of filial brain and neurobehavioral functions of the filial rats were observed. Results The lactational survival rate（81.80%：78.60% ） in both treated groups was decreased significantly（ P 〈 0.01） compared with that in the control group.Tbe body weight of filial rats on PND 10,21 in 6.70 mg/kg DM treated group[ （16.62 ± 2.2 8）, （31.34 ± 6.94）g] was less than those in the control group（ P 〈 0.05）.The delayed time in the filial rats in 6.70 mg/kg group was（3.05 ± 1.20）s and the positive rates of passive escaping response in 3.35 and 6.70 mg/kg DM treated group were 22.5% and 21.5% respeetively. There was the trend of the developmental increase of the activity of filial brain NOS between PND 5 and PND 21 and the NOS activity of rat brain on PND 5 in 6.70 mg/kg group[ （0.60± 0.07）U·mg pro^-1·h^-1] was lower than that inthecontrolgroup（P〈0.05）. Conclusion Exposure to high dose of deltamethrin in lactational female rats will decrease the activity of NOS of brain and retard the neurobehavioral development of their filial rats.

关 键 词：溴氰菊酯 一氧化氮合酶 存活率 学习 记忆 

分 类 号：R715[医药卫生—妇产科学]