高效液相色谱法测定人血清中普罗帕酮的浓度  

Determination of propafenone in human serum by HPLC

作　　者：董军亚[1] 张继红[1] 焦晓红[1] 焦建杰[2] 黄体钢[3] 

机构地区：[1]武警医学院附属医院心内科,天津300162 [2]天津医科大学药理系,天津300070 [3]天津医科大学第二医院心内科,天津300211

出　　处：《武警医学》2006年第6期427-429,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的改进高效液相色谱法测定人血清中普罗帕酮的方法.方法色谱柱：shim-pack vp-ODS柱（150 mm×4.6 mm,5 μm）.流动相为甲醇：醋酸盐缓冲液：丁胺=60：39.5：0.5（v/v）,醋酸滴定pH至4.7,流速1.0 ml/min.检测波长250nm.结果最低检出限为0.125 μg/ml.日内变异系数为5.1%～8.1%,日间变异系数为7.8%～9.0%.结论该法简便可行,值得在临床检验中推广应用.Objective A HPIE method has been established for the determination of propafenone in human serum.Methods The column was shim- pack vp- ODS（150 mm× 4.6 mm,5 μm）. The mobile phase was methanol- acetate solution- butylamine （60：39.5：0.5, v/v）, pH value was adjusted to 4.7 by acetic acid. The flow rate of mobile phase was 1.0 ml/min. The detection wavelength was 250 nm. Results LOD was 0.125μg/ml.The intra-day variance was 5.1%-8.1%, and the inter-day variance was 7.8%-9.0%.Conclusion This HPLC method is practicable in clinical lab oratory for determining propafenone concentration in serum.

关 键 词：普罗帕酮 高效液相色谱法 血药浓度 

分 类 号：R286.0[医药卫生—中药学] R972.2[医药卫生—中医学]