短期低钙饮食对去卵巢大鼠股骨生物力学特性的影响  

Effect of short-term dietary calcium deficiency on biomechanical properties of femurs in ovariectomized rats

作　　者：张岩[1] 黎韵萍[2] 周鸿奇[2] 吴春福[1] 黄文秀[2] 

机构地区：[1]沈阳药科大学药学院,沈阳110016 [2]香港理工大学

出　　处：《中国骨质疏松杂志》2006年第3期253-257,共5页Chinese Journal of Osteoporosis

基　　金：香港特别行政区科技研究发展计划(AOEP1001)项目资助课题(GYD53)

摘　　要：目的考察短期低钙饮食对去卵巢大鼠股骨生物力学特性的影响。方法大鼠假手术或去卵巢处理5周后,按照饮食中钙含量不同,分作4组:假手术常钙饮食组(SN);假手术低钙饮食组(SL);去卵巢常钙饮食组(ON);去卵巢低钙饮食组(OL)。设C组为正常对照组。分食喂养两周,收集尿液,腹主动脉取血,收集股骨,低温保存。对股骨中段进行三点弯曲生物力学实验,并通过Westernblotting检测近端股骨蛋白表达。结果双因素方差分析表明,去卵巢可显著提高血清ALP水平(P<0.001)和尿液DPD水平(P<0.01),并降低股骨弹性模量(P<0.05)和材料韧性(P<0.05)等材料力学特性。单独短期低钙饮食因素对股骨生物力学影响不显著,但去卵巢大鼠配以低钙饮食后(OL组),可显著降低股骨硬度、能量吸收、韧性等生物力学指标。低钙饮食组大鼠近端股骨维生素D受体(VDR)蛋白表达提高。结论大鼠去卵巢后体内呈现高骨转换状态;短期低钙饮食可加重大鼠由于去卵巢后股骨生物力学性能的损害;低钙饮食对骨矿代谢有一定影响。Objective To study the effect of short-term dietary calcium deficiency on the biomechanical properties of femurs in ovariectomized rats. Methods Sham operated and OVX rats were divided into four groups based on the dietary calcium level 5 weeks after the operation：Sham ＋ NCD（ normal calcium diet） （SN） ; Sham ＋ LCD （low calcium diet） （SL）; OVX ＋ NCD （ON）; OVX ＋ LCD （OL）. Another group （C） was taken as normal control. Two weeks later, urine specimens were collected and blood was taken from the abdominal aorta. Femurs were collected and stored at - 20℃ for three-point bending test in the diaphysis. Protein expression in proximal femur was detected by Western blot. Results Two-way ANOVA anslysis showed that the levels of serum ALP （ P 〈 0.001） and urine DPD （P 〈 0.01） were significantly elevated after OVX, and femoral elastic modulus （P 〈 0.05） and toughness （ P 〈 0.05 ） was reduced. Significant decrease of biomechanical markers, stiffness, energy absorption and toughness was observed in OL group, but not in those with short-term low calcium intake only. Vitamin D receptor （VDR） expression of proximal femur in LCD group was higher than that in NCD group. Conclusions Active bone turnover is observed in ovariectomized rats which can be aggravated by short-term dietary calcium deficiency. Calcium restriction has certain influence on bone mineral metabolism.

关 键 词：去卵巢 低钙饮食 股骨 生物力学 

分 类 号：R318.01[医药卫生—生物医学工程] R681[医药卫生—基础医学]