小肠RNA对^(60)Coγ射线照射后小鼠肠黏膜屏障及细菌移位的影响  

Effect of intestinal RNA on intestinal mucosal barrier and bacterial translocation in mice after abdominal γ-irradiation

作　　者：苏晓明[1] 曾桂英[1] 任东青[1] 周晓光[1] 方恒虎[1] 赵涛[1] 金成[1] 杨家骥[2] 

机构地区：[1]第四军医大学军事预防医学系放射医学教研室,西安710032 [2]第四军医大学军事预防医学系电镜中心,西安710032

出　　处：《中华放射医学与防护杂志》2006年第3期218-221,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目(30370440)

摘　　要：目的探讨小肠RNA对受γ射线照射小鼠肠黏膜屏障及细菌移位的影响。方法选用BALB/c雄性小鼠28只,用11·50Gy60Coγ射线进行腹部一次照射,于照后1~3h采用局部肠腔扩张注入法给小鼠空肠肠腔内注入正常大鼠小肠RNA,于照后5d活杀,取空肠段、肠系膜淋巴结,进行肠黏膜组织形态的观察、肠腺存活率及细菌移位率的测定。所有数据经SPSS13·0统计处理。结果(1)小肠RNA可明显提高腹部照射小鼠空肠的肠腺存活率(P<0·01);(2)光镜和扫描电镜结果显示照射对照组小肠绒毛萎缩、塌陷,黏膜下水肿,肠腺结构破坏,部分细胞变性坏死,RNA组肠黏膜形态结构明显好于照射对照组;(3)小肠RNA可降低受照射小鼠肠道细菌移位率。结论小肠RNA可改善小鼠肠黏膜屏障功能,减少细菌移位。Objective To study the effects of intestinal RNA on intestinal mucosal barrier and bacterial translocation in mice after abdominal γ-irradiation, Methods Twenty-eight BALB/c male mice were abdominally irradiated with 11.50 Gy ^60Co γ-rays, and then injected with intestinal RNA on jejunum within 3 hours after the irradiation. All the mice were sacrificed on the 5 d after irradiation. Changes of intestinal mucosal histomorphology were observed with light microscope and scanning electron microscope, and then crypt survival rate and bacterial metathetic rate were calculated ,The experimental data were analyzed with SPSS 13.0. Results Intestinal RNA significantly increased the crypt survival rate of jejunum in mice after irradiation （P〈0.01 ）, Intestinal villous atrophy and collapse, submucosal edema, and necrosis of crypts were observed in the irradiated control group. Intestinal mucosal morphosis was significantly improved in the RNA group compared with that in the irradiation control group. Intestinal RNA decreased the intestinal bacterial metathetic rate. Conelusion Intestinal RNA can improve intestinal mucosal barrier and decrease intestinal bacterial metathetic rate in mice after irradiation.

关 键 词：小肠RNA 电离辐射 肠腺存活率 肠黏膜屏障 细菌移位 

分 类 号：R818[医药卫生—放射医学]