造血因子治疗中子急性放射病的实验研究  

Treatment of acute radiation syndrome induced by neutron exposure with rhIL-11 and rhG-CSF in beagles

作　　者：王欣茹[1] 黄海潇[1] 熊国林[1] 谢玲[1] 余祖胤[1] 王宝勤[1] 董书魁[2] 罗庆良[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]北京第二炮兵总医院检验科

出　　处：《中华放射医学与防护杂志》2006年第3期221-224,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：全军医学科研"十五"计划指令课题资助项目(01L019)

摘　　要：目的通过观察造血生长因子-重组人白细胞介素-11(rhIL-11)和重组人粒细胞集落刺激因子(rhG-CSF)联合应用的方法对中子急性放射病狗的治疗效果,探讨中子损伤治疗的救治方法。方法14只雄性成年比格狗随机分为照射对照和rhIL-11+rhG-CSF治疗组。所有动物均接受90%裂变中子照射,治疗组于照后当天开始每天1次皮下注射rhG-CSF10μg·kg-1·d-1和rhIL-1150μg·kg-1·d-1,给药时间分别为14和21d。照射后观察动物的外周血象、外周血CD4+/CD8+比值变化、骨髓CFU-E及组织病理学改变。结果照射后早期对照组动物全部发生不同程度的呕吐,治疗组仅1只动物呕吐。照射后30d照射对照组死亡3只,治疗组动物全部存活。照后治疗组外周血白细胞数和恢复期血小板数明显高于照射对照组。照射后1、21和33d治疗组外周血CD4+/CD8+比值分别为照射对照组1·24、1·55和2·15倍。照射后1和33d治疗组动物骨髓CFU-E分别为照射对照组的5·25和2·34倍(P<0·01)。结论rhIL-11+rhG-CSF联合给药可明显促进中子照射所致骨髓型急性放射病狗的造血和免疫功能恢复,为细胞因子应用于中子急性放射病的临床治疗提供了实验依据。Objective To examine the therapeutic effect of combined administration of rhlL-11 and rhGCSF on acute radiation syndrome induced with neutron exposure in beagles. Methods Fourteen male beagles were randomly divided into rhlL-11 ＋ rhG-CSF treatment group （n = 7） and the control group （n = 7）. All the beagles in beth groups were whole-body irradiated with neutron of 2.0 Gy （Neutron：gamma rays = 9：1 ）. The animals in the treatment group had been subcutaneously administered daily with rhG-SCF of 10 μg· kg^-1. d^-1 and rhlL-11 of 50 μg· kg^-1 ·d^-1 for 14 and 21 days, respectively. AH the animals were killed on 33 d after the irradiation. Hemogram of peripheral blood, ratios of CD4^＋/CD8^＋, and formation of bone marrow CFU-E were used to evaluate the therapeutic effect. Results By 30 days since the neutron exposure, all the beagles were survived in the treatment group,whereas 3 beagles were died in the control group.The counts of white blood cells during the whole period of experiment and the counts of blood platelets during the recovery period were higher in the treatment group than that in the control group. The ratio of CD4^＋/CD8^＋ in the treatment group was 1.24, 1.55, and 2.15 times higher than that in the control group on the first, 21st and 33rd day since neutron exposure, respectively, More formations of CFU-E in bone marrow were observed in the treatment group,5,25 and 2.3 times more CFU-E were formatted than that in the control group on the first day and 33rd day, respectively. Conclusion The combined administration of rhlL-11 and rhG-CSF significantly improved the recovery of hematopoietic and immunological function of neutron-irradiated beagles.

关 键 词：中子 重组人白细胞介素-11 重组人粒细胞集落刺激因子 比格狗 实验治疗 

分 类 号：R818[医药卫生—放射医学]