精浆免疫抑制物质对小鼠巨噬细胞功能的影响  

Effect of seminal plasma inhibition meterial (SPIM) on the function of macrophage in mice

作　　者：王辉 李进芬 李平法 孙清河 岳汉军 

出　　处：《新乡医学院学报》1996年第3期210-212,共3页Journal of Xinxiang Medical University

基　　金：河南省卫生厅资助

摘　　要：目的探讨精浆免疫抑制物质（ＳＰＩＭ）对小鼠免疫功能的影响方法从正常男性精液中提取精浆免疫抑制物质．从ＢＡＬＢ／Ｃ雄性小鼠收集腹腔巨噬细胞（Ｍ）．通过Ｍ－ＥＡ花环试验和Ｍ—ＹＣ花环试验观察ＳＰＩＭ不同剂量及不同作用时间对小鼠腹腔Ｍ表面的Ｆｃ受体（ＦｃＲ）和Ｃ３ｂ受体（Ｃ３ｂＲ的影响．结果ＳＰＩＭ作用２４ｈ，小鼠腹腔ＭＥＡ和ＹＣ花环形成率分别为４２±４．６３和４０±５．３９，而７２ｈ分别为７４±７．９６和５９±３．４３．ＳＰＩＭＩ１５０μｇ组的ＥＡ和ＹＣ花环形成率分别为４２±１．７２和３９±２．６５，对照组则为７０±２．１３和７２±２．８７．结论ＳＰＩＭ对小鼠腹腔Ｍ的ＦｃＲ和Ｃ３ｂＲ的抑制作用有剂量依赖关系，并且以作用２４ｈ后其抑制作用达到高峰。Objective To research the effect of seminal plasma inhibitaion meterial (SPIM) on the function of immune in mice.Methods The SPIM was extractedg from the seminal fluid of momal men, and the peritoneal macrophage (M) wae exracted from the male BaIb/c mice. The effects of SPIM on surface PcR and C3bR of peritonal macroplage of mice in different time, and different dosage were observated.Rcsulcs The effects of SPIM in different time shown that the rates of / 24h troup and 72h group are 42±4.63 %and 40 ±5. 39%, 74±7.96 and 59±3. 43reepectively. The rossete forming rates of EA and y-c in SPIM 150μg group are 42±1 .72%and 39±2.65%, the rates in contyol group are 70±2. 13 and 72±2.87Conclusion The effects of SPIM on peritonal macrophage in mice have dosage-effect relationship and the suppression reached its peak after 24h.

关 键 词：巨噬细胞 FC受体 C3B受体 小鼠 SPIM 

分 类 号：R392[医药卫生—免疫学]