补肾健脾养血活血法对亚急性衰老大鼠脑组织生物化学及海马超微结构的影响  

The effect of the rule for reinforcing kidney and spleen,nourishing blood and accelerating the circulation of blood on the neurobiochemistry in brain and ultramicrostructure of hippocampus in subacute aging rats

作　　者：张小如[1] 张艳[2] 叶苓[1] 王正引[1] 

机构地区：[1]福建中医学院药学系,福建福州350108 [2]山东中医药大学基础医学院

出　　处：《中国老年学杂志》2006年第6期804-806,共3页Chinese Journal of Gerontology

基　　金：福建省自然科学基金资助项目(C9910015)

摘　　要：目的通过观察补肾健脾养血活血方对衰老大鼠脑组织神经生物化学以及海马超微结构的影响,研究该法抗脑衰老的作用机制。方法用12·5mg/ml D-半乳糖给大鼠颈背部皮下注射连续40d,形成亚急性衰老模型,检测脑组织NOS、NO、GSH-Px、MAO-B,在透射电镜下观测海马CA1区超微结构的变化。结果给药组与模型对照组比较,NO、GSH-Px显著升高(P<0·05),NOS、MAO-B显著降低(P<0·05)。给药组大鼠海马CA1区细胞核、粗面内质网、线粒体、高尔基体、脂褐素体等细胞器较模型组有明显变化。结论补肾健脾养血活血法抗脑衰老作用机制可能为通过抑制NOS活性,对机体的抗氧化系统起良性调节作用,提高GSH-Px活性,增强脑组织清除自由基的能力,抑制脑组织MAO-B活性,提高单胺类神经递质含量,增加大鼠脑组织NO含量,改善衰老大鼠海马的超微结构。Objective To explore the antiaging mechanism of the the rule for reinforcing kidney and spleen,nourishing blood and accelerating the circulation of blood by studying the effect on the neurobiochemistry in brain and ultramicrostructure of hippocampus in subacute aging model rats. Methods Animal models with experimental senescence were prepared by hypodermic injection with 12. 5 mg/ml D- gal at nape for 40 days. NOS,NO,GSH-Px,MAO-B in the brain were detected and the ultramicrostructure in CA1 of hippocampus were observed by the transmission electric microscope. Results NO and GSH-Px significantly increased （P 〈 0. 05）, NOS and MAO-B significantly decreased （P 〈 0. 05 ） in prescription group than those in model control group. The organelle including karyon, rough endoplasmic reticulum, chondriosome,dictyosome and lipofuscin in CA1 region in hippocampus of prescription group had more obvious change than those in model control group. Conclusions The rule for reinforcing kidney and spleen, nourishing blood and accelerating the circulation of blood can postpone the aging of brain by inhibiting the activity of NOS and MAO-B, enhancing the activity of GSH-Px and the content of NO, and improving the ultramicro fabric of the nerve cell in quarter CA1 in hippocampus.

关 键 词：衰老 中医药疗法 神经生物化学 超微结构 

分 类 号：R285.5[医药卫生—中药学]