γ射线辐射对高羊茅种子发芽及酶活性的影响  

THE EFFECT OF γ-RAYS IRRADIATION ON SEEDS GERMINATION AND ENZYME ACTIVITY OF TALL FESCUE

作　　者：王月华[1] 韩烈保[1] 尹淑霞[1] 王金来[1] 程晓霞[1] 

机构地区：[1]北京林业大学省部共建森林培育与保护教育部重点实验室/草坪研究所,北京100083

出　　处：《核农学报》2006年第3期199-201,224,共4页Journal of Nuclear Agricultural Sciences

基　　金：国家转基因植物研究与产业化专项项目(J2002-B-006);国家"863"项目(2001AA244082)

摘　　要：用60Coγ射线辐射处理高羊茅的3个品种千年盛世、知音和猎狗5号的干种子,观测辐射对种子发芽的影响,测定种子萌发过程中的SOD、POD和PPO酶的活性。结果表明,50Gy的辐射对高羊茅种子的萌发具有促进作用,较高剂量的辐射对高羊茅种子的萌发有抑制作用,且随着辐射剂量的加大,抑制作用增强。萌发过程中3种酶的活性均随辐射剂量的增加先升高后降低。高羊茅的适宜辐射诱变剂量为150Gy。The seeds of three cuhivars of tall fescue were treated by ^（60）Co γ-rays,seeds germination, the activity of SOD ,POD and PPO during seeds germination were analyzed. Results showed that the seed germination was promoted under 50Gy irradiation, but were inhibited in higher dose irradiation. With dose increasing, the effect of inhibition was enhanced; the activity of three enzymes was increased at lower doses and declined with dose increasing. The dose of 150Gy was recommended as suitable dosage for the tall fescue irradiation mutagenesis.

关 键 词：60Coγ射照 高羊茅 种子发芽 酶活性 

分 类 号：S688.4[农业科学—观赏园艺]