培养基中糖和氮对脱毒甘薯组培苗生长的影响  

Effects of sugar and nitrogen on virus-free plantlet growth of sweet potato in vitro

作　　者：王守明[1] 胥学峰[2] 廉美兰[1] 朴炫春 朴永昱 

机构地区：[1]延边大学农学院园艺系,吉林龙井133400 [2]吉林农业科技学院,吉林吉林132101 [3]不详 [4]延吉市园林处,吉林延吉133000

出　　处：《延边大学农学学报》2006年第2期102-106,共5页Agricultural Science Journal of Yanbian University

基　　金：延大科合字(2003)第08号

摘　　要：以脱毒甘薯的茎段为材料,研究培养基中不同糖种类、糖浓度、NH4+/NO3-及总氮量对甘薯组培苗扩繁的影响.结果表明:在MS培养基添加蔗糖或白糖,NH4+/NO3-含量为30/30(mM/mM),总氮量控制在30,60 mM时对甘薯组培扩繁有利.考虑到培养基成本,脱毒甘薯培养基以MS+食用白糖50 g/L+NH4+/NO3-30/30(mM/mM)+总氮量30 mM最为适宜.将脱毒甘薯组培苗移栽到含蛭石和草炭的营养钵中进行驯化,成活率可达95%.The effects of different types of sugar, sugar with different concentrations,NH4^＋/NO3^-,and total nitrogen in culture medium on the micropropagation of sweet potatoes were studied with nodal cuttings of virus-free plantlet. The results showed that the plantlet growth was favored in the 50 g/L or 70 g/L sucrose or white sugar,30/30 （mM/mM） NH4^＋/NO3^-, and 30 mM or 60 mM total nitrogen concentration. Considering the medium cost, the medium of MS＋ 50 g/L white sugar ＋ 30/30 （mM/mM）NH4^＋/NO3^- ＋ 30 mM total nitrogen was optimal for sweet potato growth in vitro. During the acclimatization stage, the survival ratecould reach 95 % when the plantlets were transferred in pots containing vermiculite and peat.

关 键 词：甘薯 糖种类 糖浓度 NH4^+/NO3^- 总氮量 

分 类 号：S531[农业科学—作物学]