水杨酸诱导苜蓿对霜霉病抗性的研究  

Study on resistance of alfalfa to downy mildew induced by SA

作　　者：孙涛[1] 曹致中[1] 马晖玲[1] 徐广平[1] 

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2006年第3期61-64,共4页Journal of Gansu Agricultural University

基　　金：国家科技部"十五"重大科技专项(2002BA518A03)--"优质饲草高效生产关键技术集成产业示范".

摘　　要：对陇东苜蓿、大叶苜蓿、甘农3号和关中苜蓿4个感霜霉病品种进行了水杨酸喷雾试验,研究了水杨酸对苜蓿霜霉病的诱导抗性．结果表明,陇东苜蓿的诱导抗病效果最好,诱导效果可达61．8％,用不同浓度的SA诱导处理陇东苜蓿幼苗发现,SA诱导的最宜浓度为50μg／μL;SA诱导处理后,苜蓿幼苗体内过氧化物酶(POD)和苯丙氨酸解氨酶 (PAL)活性明显增强,POD、PAL在处理后第7 d、第5 d达到最大,分别为9．8(μ／g·hfw)和4．87(△OD／0．01 gfw),是对照的2．5倍和3．2倍．Four downy mildew susceptible alfalfa cultivars, Longdong, Daye, Gannong No. 3 and Guanzhong, were sprayed by salicylic acid （SA）, and the effects of SA on resistance induction were studied. The best resistance induction effect was on alfalfa Longdon, it was 61.8%. Inducing seedlings of alfalfa Longdong with SA of different concerntration, it was showed that the optimum concerntration of SA was 50μg/μL. The activities of POD and PAL in alfalfa seedlings were obviously increased and reached the maximum,9.8 u/g · hfw and 4.87 △OD/0.01 gfw ofter treated with SA for seven days and five days respectively,which was 2. 5 times and 3.2 times as much as control respectively.

关 键 词：苜蓿霜霉病 水杨酸(SA) 抗病性诱导 过氧化物酶 苯丙氨酸解氨酶 

分 类 号：S551.7[农业科学—作物学]