脱氢表雄酮对小鼠成骨细胞增殖周期与凋亡的影响  

Effects of dehydroepiandrosterone on the proliferating cycle and apoptosis of mouse osteoblasts

作　　者：王凌[1] 李大金[1] 王文君[1] 朱影[1] 

机构地区：[1]复旦大学上海医学院附属妇产科医院暨妇产科研究所生殖免疫室,上海200011

出　　处：《中华内分泌代谢杂志》2006年第3期234-237,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金(30472259);上海市科技攻关项目基金(004019061)

摘　　要：目的探讨脱氢表雄酮(DHEA)对小鼠成骨细胞(OB)增殖周期与凋亡的调控作用。方法颅骨酶解法分离OB和体外培养OB,体外模拟雌激素撤退现象,分为对照组、10^(-10)mol/L E_2组、10^(-7)mol/L DHEA组共3组,流式细胞仪分析DNA含量,Annexin V-FITC/PI双标记流式细胞仪分析细胞早期凋亡,透射电镜观察OB超微结构。结果DNA含量分析显示,DHEA组的S期和G_2/M期细胞百分率比对照组明显增加,细胞增殖指数显著增加(P＜0.01)；DHEA和E_2对OB还具有显著的抗凋亡作用(P＜0.01或P＜0.05)；透射电镜可见经10^(-7)mol/L DHEA诱导后的OB细胞器明显增多,内质网扩张,线粒体丰富等形态学改变。结论DHEA在体外可直接促进小鼠OB的增殖并抑制其凋亡。Objective To investigate the effects of dehyroepiandrosterone （DHEA） on the proliferating cycle and apoptosis of mouse osteoblasts （OB）. Methods The OB were isolated by the enzyme-digested method from calvaria of neonatal BALB/c mice and then cultured. They were treated with 10^-10 mol/L E2 or 10^-7 mol/L DHEA in vitro. The apoptosis rate and DNA content of OB were analyzed by flow cytometry, and their ultrastructure was observed by transmission electron microscope. Results DHEA increased the percentage of the S-phase and GJM phase of OB cell cycle and improved the proliferation index significantly （ P 〈 0.01 ）. E2 and DHEA were capable of inhibiting apoptosis of OB （P 〈0.05 or P 〈0.01 ）. Moreover, OB treated with 10^-7 mol/L DHEA showed more organelles,expanded endoplasmic reticulum and abundant mitochondria. Conclusion DHEA improves proliferation of OB and inhibits apoptosis of OB induced by serum deprivation in vitro.

关 键 词：脱氢表雄酮 成骨细胞 细胞周期 细胞增殖 细胞凋亡 

分 类 号：R580[医药卫生—内分泌]