胎羊体外循环中胎盘一氧化氮合酶的变化  

The changes of nitric oxide synthase in placenta during fetal cardiac bypass

作　　者：周成斌[1] 庄建[1] 张镜芳[1] 

机构地区：[1]广东省人民医院广东省心血管病研究所心外科,广州510100

出　　处：《中华胸心血管外科杂志》2006年第3期197-200,共4页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金(30300346);广东省自然科学基金(033187)资助

摘　　要：目的探讨胎羊体外循环中胎盘一氧化氮合酶(NOS)的变化。方法孕120～140d母羊8只,随机分为对照组和转流组,每组4只。对照组假手术,转流组运用离心泵和胎盘建立胎羊体外循环,转流30min。记录转流前、后胎羊平均动脉压、脐动脉流量和动脉血气值,计算胎盘血管阻力,检测胎羊血浆内皮素1(ET1)、一氧化氮(NO)和胎盘组织NOS活性,RTPCR半定量分析eNOSmRNA转录状况。结果转流组胎羊体外循环结束后胎盘血管阻力上升,转流后2h胎盘NOS活性增强,胎盘eNOS转录水平增高(P<0.05),与对照组比较差异有统计学意义(P<0.05)。转流组胎羊血浆ET1进行性增加,与对照组之间的差异也有统计学意义(P<0.05)。两组之间血浆NO的变化没有显著差异。结论胎羊体外循环中胎盘组织NOS活性增强,不能降低胎盘血管阻力,NOS的变化可能是胎盘血管阻力增高的一种代偿。Objective To study the changes of nitric oxide synthase in placenta during fetal cardiac bypass. Methods Eight pregnant ewes （gestation 120 - 140 d）were divided equally into control group and bypass group. Bypass group underwent fetal cardiac bypass with centrifugal pump and placenta. Fetal hemedynamic date and blood gas value were recorded before and after bypass. Placenta vascular resistance was calculated. Fetal serum nitric oxide （NO）, serum endothlin-1 （ ET-1 ） and nitric oxide synthase （NOS） of placenta tissue were assayed. Transcriptions of endothelial NOSmRNA in placental tissue were assayed with RT-PCR technique. Results Acidosis and pressure of oxygen decreased after bypass appeared in fetal lamb of bypass group. Placenta vascular resistance of bypass group elevated significantly （ P 〈 0.05）. Activity of NOS and transcriptions of endothelial NOSmRNA in bypass group increased simultaneously （P〈0.05）. Serum ET-1, increased remarkably in bypass group （P〈0.05）. The difference of the serums NO between two groups was of no significance. Conclusion The increase of nitric oxide synthase in placenta can not protect placental function after fetal cardiac bypass.

关 键 词：一氧化氮合酶 胎盘 体外循环 

分 类 号：R714.5[医药卫生—妇产科学]