单纯疱疹病毒抗体的酶联免疫吸附——单扫描示波极谱法测定  

Determination of Herpes Simplex Virus Antibody (HSV-Ab) by ELISA-LSP and Its Application in the Selection of Anti-HSV Monoclonal Antibodies

作　　者：郑莉[1] 宋俊峰[2] 胡荫华 

机构地区：[1]西安石油学院,西安710065 [2]西北大学,西安710069

出　　处：《西安石油学院学报》1996年第5期54-56,共3页Journal of Xi'an Petroleum Institute

基　　金：国家自然科学基金资助课题

摘　　要：报道了用辣根过氧化物酶为标记酶的ELISA—LSP法测定单纯疱疹病毒抗体(HSV—Ab).研究了测定的最适条件;测定了型共同性单克隆抗体,其灵敏度为ELISA的2倍,线性范围比ELISA宽2个滴度;对40个杂交瘤细胞系进行单克隆筛选,测出的阳性克隆率(75%)高于ELISA所测值(72.5%);对阳性克隆的分型结果与ELISA一致,此法灵敏、可靠、易实现.: It's reported that hexpes simplex virus-antibody (HSV-Ab) was determined by ELISA-LSP which used HRP as labelling enzyme. The optimum conditions for the determintion were studied. Under optimum conditions,the linear range of HSV-Ab by ELISA-LSP was determined as 1:25 to 1 : 1600 with r=0. 9814, which was higher than 1: 50 to 1 : 800 with r = 0. 9761 by ELISA. The determination limit of ELISA-LSP(1 : 3200) was two times lower than that of ELISA(1 : 1600). The selection of anti-HSV monoclonal antibodies out of 40 systems of hybrid tumor cells was made. The positive clonal ratio was 75% by ELISA-LSP, which was higher than that by ELISA. The results of type identification of positive clonals by the above two methods are in good agreement.

关 键 词：极谱法 电化学 痕量分析 酶联免疫吸附 

分 类 号：R446.6[医药卫生—诊断学] R373[医药卫生—临床医学]