过氧化氢介导烟草叶片光合功能衰退的研究  

Photosynthetic function decline induced by hydrogen peroxide in tobacco leaves

作　　者：江力[1] 张荣铣[2] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]南京农业大学生命科学院,江苏南京210095

出　　处：《安徽工程科技学院学报（自然科学版）》2006年第2期8-12,共5页Journal of Anhui University of Technology and Science

基　　金：国家重点基础研究专项经费资助项目(G1998010100)

摘　　要：研究了不同浓度H2O2处理下烟草(Nicotiana tabacumL.cv NC89&JYH)光合功能的衰退.结果表明,0.1 mmol/L1、mmol/L H2O2对烟草离体叶片基本无影响,10 mmol/L、100 mmol/L H2O2对烟草离体和连体叶片的光合作用均有不同程度的抑制作用.H2O2处理连体叶片时类囊体光系统Ⅰ(PSⅠ)、光系统Ⅱ(PSⅡ)、全电子传递活性表现出相应的下降,H2O2处理离体叶片后,叶绿素含量的下降与内源H2O2水平上升显著相关.NC89和JYH对H2O2处理的敏感期均在叶片光合功能开始出现衰退时.Effects of H2O2 of various concentration on the photosynthetic function decline of tobacco（Nicotiana tabacum L. cv NC89 ＆ JYH）were investigated. Photosynthesis in vitro leaves was slightly influenced by 0.1,1 mmol/L H2O2 treatment and photosynthesis in vivo and in vitro leaves was inhibited in varying degrees by 10, 100 mmol/L H2 02 treatment. Thylakoid membrane photosystem Ⅰ （PS Ⅰ ）,photosystem Ⅱ （PS Ⅱ ）and whole electron transport activity in vivo declined correspondingly with H2O2 treatment. In vitro tests the decreased chlorophyll content was significantly correlated with increased endogenous H2O2 content. Sensitive phase to H2O2 treatment coincided with the beginning of photosynthetic function decline in NC89 and JYH.

关 键 词：过氧化氢 光合功能衰退 烟草叶片 

分 类 号：Q945[生物学—植物学]