正交法优化虎杖中白藜芦醇苷提取工艺的研究  

Study on Extraction Process of Piceid from Polygonum Cuspidatum by Orthogonal Design

作　　者：许汉林[1] 孙芸[1] 邵继征[1] 熊利容[1] 

机构地区：[1]湖北中医学院药学院,湖北武汉430065

出　　处：《湖北中医学院学报》2006年第2期29-30,共2页Journal of Hubei College of Traditional Chinese Medicine

基　　金：湖北省卫生厅科技基金项目;[2005JX2B67]

摘　　要：目的考察虎杖白藜芦醇苷的最佳提取分离工艺。方法采用正交实验优选提取分离条件,HPLC测定含量,丙酮重结晶纯化。结果醇提工艺的最优条件为:70%乙醇12倍量,回流提取3次,2h/次。结论该实验确定的最佳提取工艺稳定性好且简便易行。Objective： To study the optimum extraction process of piceid in polygonum cuspidatum. Methods： The optimum extraction process was investigated by orthogonal design and the determination method of piceid content was HPLC. The method that piceid was purificated by the recrystalization. Results： The optimum condition of ethanol extraction： 12 times amount of 70% ethanol and extracted 3 times, 2 hours one time. Conclusion： The optimum extraction process is stable and easy.

关 键 词：正交实验法 白藜芦醇苷 提取 分离工艺 

分 类 号：R283.6[医药卫生—中药学]