无核白鸡心葡萄组培快繁技术研究  

Fast propagation of 'Thompson seedless' by tissue culture

作　　者：李凤光[1] 娄汉平[1] 吴丽敏[1] 

机构地区：[1]辽宁省铁岭农业职业技术学院园艺系,辽宁铁岭112001

出　　处：《北方果树》2006年第4期23-24,共2页Northern Fruits

摘　　要：该试验采用无核白鸡心葡萄秋季副梢的茎段为外植体,最佳的取材时间为9月中旬,适宜的基本培养基为B5+6-BA2.0mg/L+IBA0.5mg/L+3%蔗糖+0.6%琼脂,pH值调至5.8～6.0,为无核白鸡心葡萄茎段离体培养的最佳启动萌芽培养基;B5+IBA0.5mg/L+3%蔗糖+0.6%琼脂,pH值调至5.8～6.0,为最佳增殖生根培养基,生根率达98%。试管苗根长3～4cm时开始移栽,移栽成活率90%。Stem sections of side autumn shoots of ＇Thompson seedless＇ grape as in vitro plants adopted in middle September were cultured.The suitable culture medium B5＋6-BA 2.0 mg/L＋IBA0.5 mg/L＋3% suger＋0.6% agar,pH was regulated 5.8-6.0. It was the best medium for in vitro culture of grape stem section for bud initiation. The medium of B5＋IBA0.5 mg/L＋3% suger＋0.6% agar,pH was regulated 5.8-6.0 was the best for rooting culture and rooting rate could reached 98%.Tube seedlings could be transplanted when they were 3-4 cm long and transplanted survival rates could reach 90%.

关 键 词：无核白鸡心葡萄 组织培养 快繁 

分 类 号：S663.1[农业科学—果树学]