高效液相色谱法同时测定四数獐牙菜中4种有效成分含量  

Simultaneously Determination of Four Effective Components in Swertia tetraptera Maxim.by HPLC

作　　者：梁永欣[1] 林鹏程[1] 卢永昌[1] 潘国庆[1] 

机构地区：[1]青海民族学院化学系,青海西宁810007

出　　处：《安徽农业科学》2006年第10期2163-2164,共2页Journal of Anhui Agricultural Sciences

摘　　要：建立了反相高效液相色谱法同时测定四数獐牙菜中的落干酸、獐牙菜苦苷、龙胆苦苷、芒果苷含量的方法。测定方法:在检测波长254 nm、柱温30℃、流速1 ml/min时,用ZORBAX SB-C18(250 mm×4.6 mm i.d.,5μm)柱,以甲醇和水(含0.04%H3PO4)在0～20min内由20%至35%线性梯度洗脱。试验结果:4种成分均达到基线分离,落干酸、獐牙菜苦苷、龙胆苦苷、芒果苷的线性范围分别为0.05～0.625μ(g r=0.999 9)、0.095～2.9μ(g r=0.999 8)、0.049～2.56μ(g r=0.999 7)、0.006～2.8μ(g r=0.999 9);回收率分别为101%(RSD=4.3%)、98.7%(RSD=3.3%)、99.5%(RSD=3.5%)、102%(RSD=1.0%)。结论:测定方法快速,结果准确、可靠。To establish a quantitative method of simultaneously determination of loganie acid,swertiamarin,gentiopieroside and mangiferin in Swertia tetraptera Maxim. by RP-HPLC. Method： The sample were separated on the column of Zorbax SB-C 18 （250 mm×4.6 mm i.d., 5μm ） which eluted with methanol and water （0.04 % phosphoric acid ）. The ratio of methanol and water increased from 20：80 to 35：65 in 20 rain with detected wavelength at 254 nm,flow rate at l ml/min,eolumn temperature at 30 ℃. Results： Four compounds were base-isolated. The linear ranges of loganic acid, swertiamarin, gentiopieroside and mangiferin were 0.05-0.625 μg（r=0.999 9 ）, 0.095-2.9μg（r=0.999 8 ）,0.049-2.56μg （r=0.9997） ,0.006-2.8μg （r=0.999 9）respectively,the average recoveries were 101%（RSD=4.3 % ）,98.7 %（RSD=3.3 %）,99.5 %（RSD=3.5 % ）, 102 %（RSD= 1.0 % ）respectively. Conclusions： the method is rapid and precise.

关 键 词：反相高效液相色谱 落干酸 獐牙菜苦苷 龙胆苦苷 芒果苷 四数獐牙菜 

分 类 号：S647[农业科学—蔬菜学]