硫酸耐尔蓝共振光散射法测定羧甲基纤维素钠  

Resonance Light Scattering Method for the Determination of Sodium Carboxymethyl Cellulose with Nile Blue Sulphate

作　　者：孔玲[1] 刘绍璞[1] 刘忠芳[1] 

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《应用化学》2006年第7期708-712,共5页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(20175018和20475045)资助项目

摘　　要：用共振瑞利散射（RRS）、二级散射（SOS）和倍频散射（FDS）测试技术研究了硫酸耐尔蓝（NBS）在pH值为6．0左右的Britton-Robinson缓冲溶液中，与羧甲基纤维素钠的结合反应。结果表明，该反应能导致RRS及SOS和FDS急剧增强，最大散射峰分别位于298nm（RRS）、500nm（SOS）和392nm（FDS）。羧甲基纤维素钠质量浓度在5．0×10^-8-3．0×10^-6g／mL（RRS）和5．0×10^-8-2．5×10^-6g／mL（SOS和FDS）范围内，与散射强度成正比。检出限分别为1．8×10^-9g／mL（RRS和SOS）和3．8×10^-9g／mL（FDS）。用3种方法对合成水样和烟丝提取液中羧甲基纤维素钠的含量进行了测定，结果令人满意。The resonance Rayleigh scattering （RRS）, second-order scattering （SOS） and frequency-double scattering（FDS） methods were used to study the interaction of NaCMC with nile blue sulphate in a pH 6.0 Britton-Robinson buffer solution. The results showed that the reaction could result in strong enhancement of RRS, SOS and FDS. The maximum peaks were located at 298 nm （RRS）, 500 nm （SOS） and 392 nm （FDS）. Three scattering intensities were proportional to the concentration of NaCMC in the range of 5.0 × 10^-8～3.0 × 10^-6 g/mL（RRS）, 5.0 × 10^-8～2. 5 × 10^-6 g/mL（ SOS and FDS）. The detect limit was 1.8 × 10^-9 g/mL for RRS and SOS and 3.8 × 10^-9 g/mL for FDS. The three methods were applied to the determination of the concentration of NaCMC in synthetic samples and tobacco extracts with satisfactory results.

关 键 词：羧甲基纤维素钠 硫酸耐尔蓝 共振瑞利散射 共振非线性散射 

分 类 号：O657.6[理学—分析化学]