温度和pH值对嗜水气单胞菌毒力基因表达的影响  

Effects of Temperature and pH on Expression of Virulence Factors from Aeromonas Hydrophila

作　　者：胡靖[1] 李爱华[2] 胡成钰[1] 朱大玲[2] 蔡桃珍[2] 汪建国[2] 司亚东[2] 

机构地区：[1]南昌大学生命科学院,江西南昌330047 [2]中国科学院水生生物研究所,湖北武汉430072

出　　处：《南京理工大学学报》2006年第3期375-380,共6页Journal of Nanjing University of Science and Technology

基　　金：中国科学院水生生物研究所知识创新前沿项目(220317)

摘　　要：为了解培养温度和pH值对嗜水气单胞菌的毒力基因表达以及致病性的影响,利用反转录PCR(RT-PCR)对4种基因的表达进行了研究。研究结果显示嗜水气单胞菌毒力基因的表达受温度和pH值影响,其中溶血素(AHH)、气溶素(AerA)、外膜蛋白(OMP)和粘附素(Aha)毒力基因在15℃、25℃和37℃下均能高效表达,在4℃AHH和AerA两基因也能持续表达,但OMP和Aha两基因的表达停止。在中性或偏酸性的条件下(pH5.0和pH7.0)4种毒力基因都得以表达,在碱性条件下(pH9.0)4种基因中只有AHH得以表达,而Aha等其它3个基因的表达减弱或停止。基因表达的研究结果得到鲫鱼攻毒试验结果的支持。此外,研究还表明细菌的毒力与鱼的体温也有关系。The effects of temperature and pH on the expression of four virulence factors ： hemolysin （ AHH）, aerolysin （ AerA ）, outer membrane proteins （OMP） and adhesion （ Aha）, from Aeromonas hydrophila and on its pathogenicity are studied. The results show that the expression of these virulence factors from A. hydrophila is affected by temperature and pH in the media. All four virulence factors are highly expressed in A. hydrophila when being grown at 15℃, 25 ℃ and 37℃ determined by RT-PCR. At 4 ℃, both AHH and AerA are still expressed, but neither OMP nor Aha was. All four virulence factors tested here are expressed in bacterial cells when being grown at pH 5.0 and pH 7.0. Only AHH expression is detected at pH 9.0. These results are supported by the results from the challenge experiments with crucian carp. In addition, the study shows that the pathogenicity of the bacteria is affected by the body temperature of fish.

关 键 词：嗜水气单胞菌 毒力基因 温度 PH 表达 

分 类 号：S941[农业科学—水产养殖] Q78[农业科学—水产科学]