舟山群岛近海底栖真菌及其抗生活性初筛研究  

Study on Benthal Fungi from Sediments of Zhoushan Archipelago and Anitibiosis Screen

作　　者：王骁勇[1] 黄耀坚[1] 郑忠辉[1] 高亚辉[1] 宋思扬[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2006年第4期558-562,共5页Journal of Xiamen University：Natural Science

基　　金：国家"863"计划(2001AA620401);国家"973"计划项目(2001CB409701);福建省自然科学基金(C0210001)资助

摘　　要：对分离自舟山群岛海域海底46个沉积物样品中的丝状真菌进行种属鉴定,及其代谢产物抗菌、细胞毒和乙酰胆碱酯酶抑制活性的检测.结果显示:舟山群岛海域海底沉积物中存在着丰富的真菌资源.46个沉积物样品中,真菌含量最高可达到2 250 CFU/g(样品).在进行活性检测的89份真菌代谢产物中,有29.2%的样品对一株或一株以上的指示细菌有不同程度的抑制作用;各有10%左右的样品对5株植物致病真菌显示抑制作用,有49.4%的样品对白色假丝酵母显示抑制作用,但没有样品对黑曲霉有抑制作用;在细胞毒实验中,分别有2个、4个和12个样品在浓度为25μg/mL时,对KB细胞、Raji细胞和Hep G2细胞的抑制率高于50%;有55个样品显示微弱的乙酰胆碱酯酶抑制活性.通过此次研究,我们对舟山群岛海域的底栖真菌资源有了初步了解,将有助于将来更全面深入的研究.Fungi from 46 sediment samples of Zhoushan Archipelago were categorized,and the activity of antimicrobial,antitumor and the anti-AchE of their metabolite were tested. Results indicated：fungi resource in sediments of Zhoushan Archipelago is abundant. In 46 sediments,the highest fungi content reached to 2 250 CFU/g,and most of them were belonged to genus Penicillium and Aspergillus. According to samples they were isolated from and culture characters,89 fungi were chosen to ferment, and their metabolites were used to antibiosis test. In these metabolites,29.2 % showed inhibition to one or more indirector bacteria strains; There are 10 % samples show inhibition to each of five nosogenetic fungi strains,and 49.4 % to Candida albicans,but no one to Aspergillus niger;In cytotoxic test by MTT method, there are 2,4, 12 samples＇ inhibition rate to KB, Raji and Hep G2 respectively higher than 50% in 25 μg/mL. In anti-AchE test,though 55 samples show inhibition,their activities were very weak,and do not show inhibition below 100μg/mL. In this study,the profile of fungi resource in the Zhoushan Archipelago was obtained, and it would be aid to further research.

关 键 词：海洋真菌 抗菌活性 细胞毒活性 抑制乙酰胆碱酯酶 

分 类 号：Q939.92[生物学—微生物学]