2羟-基-4-甲氧基苯甲醛和2羟-基-4-甲氧基苯甲酸对菜粉蝶多酚氧化酶的抑制作用  

Inhibitory effects of 2-hydroxy-4-methoxy-benzaldehyde and 2-hydroxy-4-methoxy-benzoic acid on polyphenoloxidase of Pieris rapae(L.)

作　　者：王勤[1] 柯莉娜[1] 薛超彬[2] 贺量[1] 罗万春[2] 陈清西[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005 [2]山东农业大学农药毒理与应用技术省级重点实验室,山东泰安271018

出　　处：《广西农业生物科学》2006年第2期155-159,共5页Journal of Guangxi Agricultural and Biological Science

基　　金：福建省重点科技项目(2004N002);国家自然科学基金项目(30570408)

摘　　要：测定了2羟-基-4-甲氧基苯甲醛和2羟-基-4-甲氧基苯甲酸两种效应物对菜粉蝶多酚氧化酶(po lypheno lox idase,简称PPO,EC.1.14.18.1)催化L-多巴(L-DOPA)氧化活力的抑制作用。结果表明,这两种效应物对该酶的活性均有明显的抑制作用,抑制中浓度(IC50)分别为2.71和5.66 mm o l/L,抑制常数分别为2.82和3.35 mm o l/L;2羟-基-4-甲氧基苯甲醛对该酶的抑制作用显著高于2羟-基-4-甲氧基苯甲酸的;这两种抑制剂对酶的抑制作用机理完全不同,2羟-基-4-甲氧基苯甲醛对酶的作用表现为混合型抑制,而2羟-基-4-甲氧基苯甲酸对酶的作用表现为竞争性抑制。研究结果为设计以该酶为靶标的杀虫剂提供理论依据。Inhibitory effects of 2-hydroxy-4-methoxy-benza[dehyde and 2-hydroxy-4-metnoxybenzoic acid on polyphenoloxidase （PPO） from larvae of Pieris rapae （L.） were studied. The results showed that both compounds could obviously inhibit enzyme activity for oxidation of L-DOPA, and their IC50 （inhibitor concentration leading to 50% activity lost） were 2.71 and 5.66 mmol/L, respectively. Their inhibition constants were 2.82 and 3. 35 mmol/L. Inhibitory ability of 2-hydroxy- 4-methoxy-benzaldehyde was much higher than that of 2-hydroxy-4-methoxy-benzoic acid. Inhibitory mechanisms of the two compounds were very different. The inhibition of 2-hydroxy-4-methoxy-benzaldehyde was mixed type while that of 2-hydroxy-4-methoxy-benzoic acid was competitive type.

关 键 词：菜粉蝶 多酚氧化酶 2-羟基-4-甲氧基苯甲醛 2-羟基-4-甲氧基苯甲酸 抑制作用 

分 类 号：S482.3[农业科学—农药学] S436.3[农业科学—植物保护]