一种改良的大肠杆菌质粒平板转化方法  

A Modified Plate Transformation Method of Escherichia coli by Plasmids

作　　者：石琰璟[1] 杨隽娴[1] 葛晓萍[1] 

机构地区：[1]青岛科技大学化工学院,山东青岛266042

出　　处：《青岛科技大学学报（自然科学版）》2006年第3期193-195,共3页Journal of Qingdao University of Science and Technology:Natural Science Edition

摘　　要：改良了大肠杆菌质粒平板转化法,在储存液中添加PEG(聚乙二醇)、MgSO4和甘油,在-30℃保存受体大肠杆菌细胞,然后将保存菌与分子量大小不同的质粒DNA混匀,直接在Ca2+离子选择平板上进行转化和筛选。改良的平板转化法对大分子量DNA的转化无论在转化效率还是转化保持能力上都大大优于传统CaCl2转化法。A modified plate transformation method of Escherichia coli by plasmid was described. Recipient Escherichia coli cells were stored at -30 ℃ in PEG, MgSO4 and glycol solution, then plasmids with different molecular weights were mixed with these cells, and transformed and selected directly in CaCl2 plates. It was showed that the modified plate transformation method was of higher transformation efficiency and maintenance than classic CaCl2 method when transformed by plasmid of higher molecular weight.

关 键 词：转化 大肠杆菌 平板转化 PEG 转化效率 转化保持能力 

分 类 号：Q785[生物学—分子生物学]