人AITRL基因克隆和序列分析  

Molecular cloning and sequence analysis of human activation-inducible tumor necrosis factor recepter ligand gene

作　　者：毛朝明[1] 王胜军[1] 蒋茜[2] 马洁[1] 杨敏[1] 仝佳[1] 许小朋[1] 邱谷风[1] 唐莉[1] 邵启祥[1] 许化溪[1] 

机构地区：[1]江苏大学医学技术学院免疫学研究室,江苏镇江212001 [2]江苏大学附属医院核医学科,江苏镇江212001

出　　处：《江苏大学学报（医学版）》2006年第3期189-192,共4页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省自然科学基金资助项目(BK2004405);国家自然科学基金资助项目(30300169)

摘　　要：目的:克隆人AITRL基因cDNA,同时对其序列进行分析。方法:采用RT-PCR方法,从人脐静脉内皮细胞株中获得AITRL基因的cDNA,克隆至pMD18-T载体,选择阳性克隆进行酶切鉴定和序列测定。结果:扩增得到的AITRL基因cDNA为534 bp,编码177个氨基酸残基,与GenBank中公布的序列完全一致。结论:获得人AITRL基因的克隆,为进一步研究其生物学功能奠定了良好基础。Objective： To clone and analyze a full-lenth cDNA encoding human activation-inducible tumor necrosis factor receptor ligand （AITRL） gene. Methods： The eDNA of AITRL was amplified by RTPCR using the total RNA extracted from human umbilical vein endothelial cell （HUVEC） . The PCR product was inserted into pMD18-T vector and then transformed into E. coli DH5α. The positive recombinant clone was analyzed by digestion of restriction endonuelease and DNA sequencing. Results： The eDNA of AITRL has an complete open reading frame with a length of 534 bp, which encodes a product of 177 amino acid, and shares 100 % homology with the sequence of mRNA for human AITRL in GenBank. Conclusion： The eDNA of human AITRL was cloned successfully, which posed a basis for further researching on its biological function.

关 键 词：活化可诱导的肿瘤坏死因子受体配体 CDNA克隆 RT-PCR 人脐静脉内皮细胞 

分 类 号：R392[医药卫生—免疫学]