溴酸钾-亚甲兰催化分光光度法测定亚硝酸盐的研究  

Spectrophotometric Determination of Nitrite Based on Potassium Bromate-methylene Blue Catalysis

作　　者：姜爱莉[1] 刘雪梅[1] 王雪涛[1] 

机构地区：[1]烟台大学生物工程系,山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2006年第3期193-197,共5页Journal of Yantai University(Natural Science and Engineering Edition)

摘　　要：对水中亚硝酸盐测定的溴酸钾-亚甲蓝催化分光光度法进行了研究.通过单因素实验和正交实验,得出测定的最佳条件为:向比色管中加入3 mL H3PO4、3.5 mL亚甲蓝、4.0mL KB rO3溶液,置于40℃水浴中加热3 m in.取出后先加入1 mL乙酸钠中止反应,然后冷却至室温,在波长678 nm处测量吸光度.该法的测定范围为0.1-4.0 mg/mL.The spectrophotometric determination of water nitrite is studied based on potassium bromate-methylene blue catalysis. With single factor and orthogonal experiments, the optimum conditions are obtained as follows： 3.5 mL methylene blue solution, 3 mL phosphate acid ,4 mL potassium bromate and 2 mL nitrite solution are added into colorimetric tube. Heated at 40℃ for 3 minutes, then 1 mL acetate sodium solution is added to stop the reaction and the tube is cooled to room temperature, finally ence solution. The linear the percent absorbance is determined at 678 nm with water as the referrange of the result is 0. 1 to 4.0 g/L.

关 键 词：亚硝酸盐 催化分光光度法 亚甲蓝 

分 类 号：Q949.2[生物学—植物学]