藻酸双酯钠对阿霉素肾病大鼠保护作用的实验研究  

Protective effects of polysaccharide sulfate on adriamycin induced nephrosis rats

作　　者：余健[1] 郭仁寿[1] 陈重义 张龙江 刘雪梅[1] 孔生生[1] 

机构地区：[1]广州军区武汉总医院儿科

出　　处：《肾脏病与透析肾移植杂志》1996年第5期27-31,共5页Chinese Journal of Nephrology,Dialysis & Transplantation

摘　　要：目的：探讨藻酸双酯钠（ＰＳＳ）对肾病综合征（ＮＳ）的疗效及机理。方法：用阿霉素（ＡＤＲ）静脉注射建立大鼠肾病模型，观察大鼠尿蛋白排泄量，测定有关生化指标和凝血功能，并以ｐｏｌｙｅｔｈｙｌｅｎｅｍｉｎｅ（ＰＥＩ）为探针检查肾病大鼠ＧＢＭ阴离子位点，结合组织学及形态学改变，进行远期疗效评估。结果：ＰＳＳ可显著降低肾病大鼠尿蛋白的排泄，纠正脂质代谢紊乱，延缓ＭＣＮＳ发展至ＦＳＧＳ的进程，且对肾小球电荷屏障具有保护作用。结论：胃饲ＰＳＳ对阿霉素肾病大鼠具有明显的保护作用。BACKGROUND DESIGN ADR (7.5 mg/kg) was used to induce a nephrotic model in rat. PSS administration was started 7 days before adriamycin was given. Urinary protein excretion, anionic sites on GBM shown by a cationic probe polyethyleneimine (PEI) and histomorphologic changes were compared between the PSS treated nephrotic rats and the untreated control rats. RESULTS 〖WTBZ] At 14, 28 and 42 days after ADR injection, proteinuria was significantly reduced in PSS treated nephrotic rats as compared with untreated nephrotic rats ( P <0.01). After 45 days, those PSS treated nephrotic rats showed a marked attenuation in mean serum cholesterol and triglyceride concentration. Meanwhile, the anionic sites were arranged one layer on most segments of GBM in PSS treated rats; two layer arrangement could still be seen in some segments in this group, the size of PEI particles was similar to that in the normal rats. After nine months, significantly higher degrees of glomerular hypertrophy and sclerosis were seen in untreated rats than in PSS treated rats compared by quantitative morphometric analysis. CONCLUSION 〖WTBZ] The results indicated that PSS can obviously decrease urinary protein excretion, protect the charge selective barrier in glomeruli, correct disordered lipid metabolism and retard the progression from minimal change disease to focal and segmental glomerulosclerosis.

关 键 词：肾疾病 藻酸双酯钠 阿霉素中毒 

分 类 号：R692.05[医药卫生—泌尿科学]