反转录─多聚酶链反应技术定量检测人外周血单个核白细胞低密度脂蛋白受体基因的表达  

Expression Levels of Low Density Lipoprotein Receptor mRNA in Peripheral Blood Mononuclear Cells from Patients with Hyperchol esterol emia and Coronary Heart Disease by Reverse Transcription-polymerase Chain Reaction Procedure

作　　者：秦树存[1] 王士雯[1] 程云 

机构地区：[1]中国人民解放军总医院老年心内科,中国人民解放军第302医院免疫室

出　　处：《中国动脉硬化杂志》1996年第2期141-144,共4页Chinese Journal of Arteriosclerosis

摘　　要：人外周血单个核白细胞低密度脂蛋白受体的ｍＲＮＡ为低丰度表达，不易检出，本文应用反转录－多聚酶链反应技术建立的低密度脂蛋白受体基因表达定量检测法灵敏、简捷、特异。为临床观察高胆固醇血症患者和冠心病易患人群的低密度脂蛋白受体表达情况，提供了方便必要的手段，初步应用结果表明，高胆固醇血症和冠心病患者的低密度脂蛋白受体ｍＲＮＡ水平明显降低。Low density lipoprotein receptor(LDLR) mRNA in peripheral blood mononuclear cells(PBMC) belong to low abundance expression and is difficult to detect.This study found that using the reverse transcription(RT)-polymerase chain reaction(PCR)procedure for semiquantitative determinating expression levels of LDLR gene is a highly sensitive and specific,and gives a convinient and necessary method for clinical studying expression levels of LDLR gene in PBMC from patients with hypercholesterolemia and coronary heart disease.

关 键 词：低密度脂蛋白 高胆固醇血症 冠心病 受体基因 

分 类 号：R589.204[医药卫生—内分泌] R541.404[医药卫生—内科学]