用HPLC法测定止喘胶囊中淫羊藿苷和蛇床子素的含量  

Determination of icariin and cnidiadin in Zhichuan capsule by HPLC method

作　　者：李剑锋 周昕[2] 沈谦[2] 

机构地区：[1]上海南汇区中心医院药剂科,上海201300 [2]上海中医药大学附属龙华医院药剂科,上海200032

出　　处：《药学服务与研究》2006年第3期215-217,共3页Pharmaceutical Care and Research

摘　　要：目的：研究止喘胶囊中淫羊藿苷和蛇床子素含量测定的方法。方法：采用Kromasil C18色谱柱（250mm×4．6mm,5μm），流动相为甲醇-1．25％醋酸的梯度洗脱。流速1．0mL／min。室温。利用二极管阵列检测器，在270nm和322nm两个波长下同时测定止喘胶囊中淫羊藿苷和蛇床子素的含量。结果：该方法分离效果好，精密度高，加样回收率令人满意。淫羊藿苷在0．228～2．28μg间有良好的线性关系，r=0．9997；蛇床子素在0．4304．300μg之间有良好的线性关系，r=0．9993。结论：HPLC法可用于止喘胶囊的质量控制。Objective： To establish a HPLC method for determination of icariin and cnidiadin in Zhichuan capsule. Methods： A precise HPLC method was developed and validated for the simultaneous determination of icariin and cnidiadin on Kromasil Cl8 column （250 mm×4.6 mm,5 μm）. Methanol and 1.25% acetic acid water was used as mobile phase with gradient elution at a flow rate of 1.0 mL/min. The assay of icariin and cnidiadin was quantified at the temperature of 25℃ , with diode array detector, at the wavelength of 270 nm and 322 nm, respectively. Results： Good separation, high precision and a satisfactory recovery were demonstrated by the method, which was found to be linear over the range of 0. 228-2.28 μg for icariin （r= 0. 999 7）, and 0. 430-4. 300 μg for cnidiadin （r= 0. 999 3）. Conclusion： The method is suitable for quality control of Zhichuan capsule.

关 键 词：淫羊藿苷 蛇床子素 色谱法 高压液相 止喘胶囊 

分 类 号：R927.2[医药卫生—药学]