氧应激毒性产物丙二醛(MDA)对小鼠体能的影响及其体内代谢  

Toxic Effects of Oxidative Stress Product Malondialdehyde(MDA) on Mice Physical Stamina and Metabolism in vivo

作　　者：李莉[1] 陈菁菁[1] 李方序[1] 朱泽瑞[1] 廖艳阳[1] 印大中[1] 

机构地区：[1]湖南师范大学生命科学学院蛋白质化学与发育生物学教育部重点实验室,中国长沙410081

出　　处：《湖南师范大学自然科学学报》2006年第2期97-101,共5页Journal of Natural Science of Hunan Normal University

基　　金：国家自然科学基金资助项目(30070874)

摘　　要：研究了腹腔注射丙二醛（MDA）对昆明小鼠体能行为（游泳、爬杆）的影响,结合荧光分析探索了MDA在小鼠体内的代谢过程.结果发现：一次性给药或长期给药MDA对小鼠的体能影响均达到了显著水平（P＜0.05）,但MDA造成的急性影响比慢性影响更大;荧光分析表明,给药后5min是MDA吸收与代谢的阈值时间,给药后5～10min,血液中MDA浓度达到最大值,10min以后,代谢为主,给药后1 h,MDA被代谢约80%;血清、脑组织和肝脏的MDA含量分析表明：MDA的代谢途径是由血液流向肝脏,再由肝脏流向机体组织（包括脑组织）.这些结果对于了解不饱和醛酮中毒后的代谢机制,及对于氧应激疲劳和相关的体能恢复生化机理,具有重要的理论及临床指导意义.The toxic effects of MDA treatment （abdomen injection） on the physical stamina （swimming, poleclimbing） of Kun-min （KM） mice and the metabolism of MDA have been studied. The results showed that both instant injection and long-term repetitive injection of MDA （for 20 or 30 days） reduced physiological performance of mice to a significant level （ P 〈 0.05）. Acute physiological stamina decline were found more obvious than chronic effect of MDA-blood MDA concentration peak was observed after 5-10 min of MDA injection, measured with a spectral fluorometer, after 10 min, metabolism is the main process, concentration of MDA was found degraded remarkably within one hour （80%）. The MDA contents in serum, brain and liver of KM mice demonstrated that the metabolic pathway of MDA in mice was from the blood to the liver, then to other tissues （including brain tissues）. These results may help understanding the detoxification metabolism of unsaturated carbonyls following oxidative stress during tiredness and recovery in body, and provide valuable information for clinical practice in the future.

关 键 词：丙二醛 昆明小鼠 应激反应 荧光 

分 类 号：S512[农业科学—作物学] Q581[生物学—生物化学]