毛细管电泳免疫化学发光高灵敏检测人血清中肿瘤标志物甲胎蛋白  

Highly Sensitive Chemiluminescence Detection of Tumor Marker alpha-Fetoprotein in Human Sera with Capillary Electrophoresis Immunoassay

作　　者：刘彦明[1] 穆海贝[1] 王成全[1] 陈永红 李福荣[3] 王军华[4] 程介克[4] 

机构地区：[1]信阳师范学院化学化工学院,信阳464000 [2]信阳市中心医院,信阳464000 [3]信阳师范学院生命科学学院,信阳464000 [4]武汉大学化学与分子科学学院,武汉430072

出　　处：《高等学校化学学报》2006年第7期1242-1244,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20575056);河南省杰出青年科学基金(批准号:04120001300);河南省高等学校创新人才培养工程(批准号:2005126)资助

摘　　要：A novel detection method of a tumor marker alpha-fetoprotein（AFP） in human sera via capillary（electrophoresis） immunoassay coupled with chemiluminescence detection（CEIACL） was established.After a simple non-competitive immunoassay procedure,seras were directly injected into capillary for electrophoresis with chemiluminescence reaction of luminol and H2O2 catalyzed by horseradish peroxidase and enhanced by（p-iodophenol）.The detection limit（S/N=3） of AFP is 0.85 μg/L.The CEIACL method proposed was successfully applied to the sensitive detection of AFP in human sera,which coincides with the results detected by Beckman automatic CL analyzer-access IA system.A novel detection method of a tumor marker alpha-fetoprotein （AFP） in human sera via capillary electrophoresis immunoassay coupled with chemiluminescence detection （CEIACL） was established. After a simple non-competitive immunoassay procedure, seras were directly injected into capillary for electrophoresis with chemiluminescence reaction of luminol and H2O2 catalyzed by horseradish peroxidase and enhanced by p-iodophenol. The detection limit （S/N = 3） of AFP is 0. 85 μg/L. The CEIACL method proposed was successfully applied to the sensitive detection of AFP in human sera, which coincides with the results detected by Beckman automatic CL analyzer-access IA system.

关 键 词：毛细管电泳 免疫分析 化学发光检测 甲胎蛋白 

分 类 号：O657[理学—分析化学]