小分子多肽HLP核素示踪分析方法研究  

Radioisotopic Tracing Method of Hirulog-like Peptide

作　　者：钱隽[1] 唐晓峰[2] 万丹晶[1] 朱建华[1] 

机构地区：[1]复旦大学药学院放射药学教研室,上海200032 [2]上海市高血压研究所,上海200025

出　　处：《高等学校化学学报》2006年第7期1247-1249,共3页Chemical Journal of Chinese Universities

基　　金：上海交通大学医学院校级基金(批准号:02XJ21007)资助

摘　　要：The radioisotopic tracing method of Hirulog-like peptide（HLP） was established, wihch could be a reference for the pharmacokinetic analysis in small molecule polypeptide research.（）125I-HLP was prepared by Iodogen method.The radiochemical purity and stability in vitro of（）125I-HLP were determined by HPLC and SDS-PAGE.The radiochemical purity of(（）125I-HLP) was shown above 95% and was stable for 53 hours.It is the first time using SPE to separate the labeled compound from radioiodide and small molecule polypeptides.The HLP recovery was（92.60±6.31）% by SPE and the coefficient of variation was lower than 10% in a concentration range from 10 to 100 000 ng/mL.The detectable limitation reached 52.79 ng/mL.The radioisotopic tracing method of Hirulog-like peptide （HLP） was established, wihch could be a reference for the pharmacokinetic analysis in small molecule polypeptide research. 125^I-HLP was prepared by Iodogen method. The radiochemical purity and stability in vitro of 125^I-HLP were determined by HPLC and SDS-PAGE. The radiochemical purity of 125^I-HLP was shown above 95% and was stable for 53 hours. It is the first time using SPE to separate the labeled compound from radioiodide and small molecule polypeptides. The HLP recovery was （92.60 ± 6.31 ）% by SPE and the coeiffcient of variation was lower than 10% in a concentration range from 10 to 100 000 ng/mL. The detectable limitation reached 52. 79 ng/mL.

关 键 词：小分子多肽 HLP 125^I 分析方法 

分 类 号：O657.4[理学—分析化学]