具有DNA切割功能的新型多聚酰胺/丝组缀合物  

A Novel Polyamide/Ser-His Conjugate with DNA-cleaving Function

作　　者：牛明玉[1] 叶勇[2] 刘伟[1] 张祯[1] 赵玉芬[1] 

机构地区：[1]清华大学化学系生命有机磷化学及化学生物学教育部重点实验室,北京100084 [2]郑州大学化学系化学生物学和有机化学河南省重点实验室,郑州450052

出　　处：《高等学校化学学报》2006年第7期1253-1256,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20132020;20572061)资助

摘　　要：为得到具有核酸切割功能的人工核酸酶,设计合成了一种新型多聚酰胺/丝组缀合物,并研究了其DNA切割活性.合成的目标化合物在pH=6.0的BR缓冲溶液中对pBR322 DNA切割活性的初步实验结果表明,于37℃保温6 h后,pBR322 DNA基本上被完全从FormⅠ切割为FormⅡ,保温36 h后,pBR322DNA几乎被切割完全.Recently, polyamides containing N-methylpyrrole have attracted considerable attention from synthetic and biological chemists. Based on Dervan＇s theory, the polyamide/Ser-His conjugate which is a synthetic analogue of natural products for DNA affinity was synthesized through the dicyclohexylcarbodiimide/1-hydroxy- benzotriazole（DCC/HOBt） coupling reaction, and its cleavage activity for DNA was also studied. In BR buffer（pH =6. 0）, pBR322 DNA can be cleavaged by the title compound at 37 ℃. After 6 h, pBR 322 DNA was transferred from form Ⅰto form Ⅱ, after 36 h, pBR 322 DNA was cleavaged totally.

关 键 词：多聚酰胺/丝组缀合物 DNA 切割试剂 

分 类 号：O621.33[理学—有机化学]