b-FGF和TGF-β1对体外培养的软骨细胞增殖的影响  

Experimental research on proliferation of chondrocytes in vitro using TGF-β1 and/or b-FGF

作　　者：丁小邦[1] 高建华[1] 夏万尧[2] 陈兵[2] 周广东[2] 崔磊[2] 刘伟[2] 曹谊林[2] 

机构地区：[1]南方医科大学附属南方医院整形外科,广东广州510515 [2]上海第二医科大学附属第九人民医院整复外科上海组织工程研究开发中心

出　　处：《中国美容医学》2006年第6期622-624,共3页Chinese Journal of Aesthetic Medicine

基　　金：国家"973"组织工程研究项目04项子课题(G19990543004)

摘　　要：目的:研究转化生长因子-β1(TGF-β1)和碱性成纤维细胞生长因子(b-FGF)对体外培养的软骨细胞增殖的影响。方法:细胞取自猪耳弹性软骨,体外培养原代至第6代软骨细胞,将培养液中加入TGF-β1或/和b-FGF作用于软骨细胞,并用MTT比色分析法对其增殖行为进行分析,探讨两种因子对软骨细胞增殖行为的影响。结果:b-FGF明显促进原代至第3代软骨细胞的增殖,并逐渐减弱,对第4代细胞无增殖作用,TGF-β1仅促进原代软骨细胞的增殖,TGF-β1和b-FGF联合应用,其刺激细胞增殖的作用明显低于b-FGF单一作用。结论:b-FGF显著促进体外培养的软骨细胞的增殖,主要作用于原代至第2代软骨细胞,明显强于b-FGF和TGF-β1联合应用。Objective To study the effect of proliferation of porcine elastic chondrocytes in vitro using TGF-β1 and/ or b-FGF. Methods Cells were harvested from the elastic auricular cartilage of swines. To study the rule of proliferation of chondrocytes from passage 0 to passage 6 in Ham＇s F-12 media including TGF-β1 and/or b-FGF Using MTT method, Results b-FGF had an signif icant effect on proliferation of chondrocytes from passage 0 to passage 3,which disappeared in passage 4. TGF-β1 had an increasing effect on chondrocytes only in passage 0.The combination of TGF-β1 and b-FGF led to a less effect on proliferation of chondrocytes than b-FGF, Conclusion b-FGF had a significantly increasing effect on proliferation of chondrocytes from passage 0 to passage 3, which is more signicant than the effect of combination of TGF-β1 and b-FGF.

关 键 词：转化生长因子-β1(TGF-β1) 碱性成纤维细胞生长因子(b-FGF) 软骨细胞 

分 类 号：Q813.11[生物学—生物工程]