致敏IEL过继转移诱导抗弓形虫感染的机制  

Adoptive transfer of primed IEL induces T cellular immunity in Peyer’s Patches and the protection against Toxoplasma gondii infection

作　　者：杨亚波[1] 沈嬿琼[1] 殷国荣[1] 刘红丽[1] 

机构地区：[1]山西医科大学医学寄生虫学研究所,山西太原030001

出　　处：《中国病原生物学杂志》2006年第3期198-201,共4页Journal of Pathogen Biology

基　　金：山西省自然科学基金项目(No.20041105);山西省高校科技研究开发项目(No.20041238);山西医科大学基础医学院"学科研究方向建设基金"(No.20040708)

摘　　要：目的分离供体鼠感染弓形虫后不同时间点的小肠上皮内淋巴细胞(intraepithelial lymphocyte,IEL)并过继转移,研究其诱导受体鼠抗弓形虫感染作用及其机制。方法BALB/c小鼠经口感染弓形虫速殖子5×104个/只,对照组未感染,作为供体提供IEL。同品系小鼠36只为受体鼠,分为6组,每组6只,经尾静脉分别接受分离自供体鼠感染弓形虫后第7(IEL7组)、9(IEL9组)、11(IEL11组)、13(IEL13组)、15d(IEL15组)的致敏IEL或未致敏(IEL0组,即对照组)的IEL3×105/0.2ml·只。各组小鼠过继转移后第4d,用弓形虫速殖子灌胃攻击,攻击后第13d分离纯化脑、肺、脾组织内速殖子并计数,测定小肠Peyer′s Patch(PP)CD4+、CD8+T细胞亚群水平。结果致敏IEL过继免疫可使受体鼠脑、肺、脾组织内速殖子数显著减少,接受致敏后第13d IEL的小鼠组织内速殖子数减少最为显著(Ρ<0.01),与IEL0组相比,脑、肺和脾组织内速殖子数分别减少81.13%、58.43%和70.97%;同时,小肠PP结CD4+T细胞增多,CD4+/CD8+比值增高(Ρ<0.01)。结论过继转移致敏IEL能显著提高受体鼠的抗弓形虫感染能力,且保护作用同致敏时间关系密切,以感染后13d分离的致敏IEL保护性最强。Objective To determine the mucosal immune responses and protection against challenge in mice following in- traepithelial lymphocyte （IEL） adoptive transfer which separated from mice orally infected with To-roplasma gondli at certain times. Methods BALB/c mice donor provided IEL after orally infected 5 ×10^4 tachyzoites or no infection. Thirty-six recipient mice were divided into 6 groups named IEL7, IEL9, IEL11 IELl5 andIELl5, which received primed IEL 3 × 10^5via tail vein purifying on days 7, 9, 11, 13 and 15 postinfection （p. i. ）, respectively, and IELo, receiving unprimed IEL. Four days after adoptive transfer, each mouse was challenged with 5 ×104 tachyzoites by intragastric administration. On day 13 post challenge, the tachyzoites in brain, lung and spleen were purified and counted and the T lymphocyte sub- sets in Peyer＇s Patches were detected. Results Primed IEL adoptive transfer decreased tachyzoites burden in brain, lung and spleen, especially the tachyzoites burden in IELls decreased remarkably （P〈 0.01）, which decreased 81. 13% in brain, 58. 43% in lung and 70.97% in spleen. In Peyer＇s Patches, the average percentage of CD4＋ T cells and CD4＋/ CD8＋ ratio increased. Conclusion Primed IEL can significantly elevate the anti-infection ability of recipient mice a- gainst T. gondii, and the protection effect has intimate relationship with sensitize time. Adoptive transfer primed IEL which was purified on day 13 p.i. provided the greatest protection for recipient mice.

关 键 词：弓形虫 粘膜免疫 过继转移 Peyer’s Patch小鼠 

分 类 号：R382.5[医药卫生—医学寄生虫学]