大鼠脂肪条带结构针刺镇痛效应特异性的生物学基础  

Biological basis of specifical analgesia effect following fatty strap structure acupunctured in rats

作　　者：张俊芳[1] 孟凡迅[2] 党瑞山[3] 刘芳[3] 陈尔瑜[3] 周鸣鸣[4] 王军[4] 陈磊[4] 瞿前进[4] 

机构地区：[1]南通大学附属医院眼科研究所,江苏南通226001 [2]南通大学神经再生重点实验室,江苏南通226001 [3]上海第二军医大学人体解剖教研室,上海200433 [4]南通大学生命科学学院,江苏南通226007

出　　处：《四川中医》2006年第7期21-22,共2页Journal of Sichuan of Traditional Chinese Medicine

基　　金：上海市科学技术发展基金攻关项目(014319365)江苏省教育厅自然科学研究基金资助项目(编号:04KJB360115)

摘　　要：目的:观察大鼠脂肪条带结构针刺镇痛效应特异性的生物学基础。方法:选择大鼠胃经沿线脂肪条带结构上后三里以及脂肪条带结构旁两个对照位点(A1、A2),分别进行针刺,针刺结束后立即断头处死,分离出胃经沿线脂肪条带结构,制备匀浆液,采用放免法测定样本中的β内啡肽(βEP)、强啡肽A(DynA)、P物质(SP)含量。结果:βEP针刺后三里(19.08±7.42)与针刺A1、A2位点(2.27±1.99)经成组t检验比较,两者差异显著(t=5.5374,P<0.01)。DynA针刺后三里(23.08±8.61)与针刺A1、A2位点(2.19±1.81)经成组t检验比较,两者差异显著(t=5.8194,P<0.01);SP针刺后三里(1.57±0.49)与针刺A1、A2位点(1.05±0.60)经成组t检验比较,两者差异不显著(t=1.6330,P>0.05)。结论:针刺正常大鼠后三里腧穴,可使胃经沿线脂肪条带结构上特异性针刺镇痛效应物质βEP、DynA含量升高。Objective： To study the biological basis of specifical analgesia effect by acupuncturing fat-stripe - like structure in rats. Methods： Tsu san li （st 36 ） was acupunctured on the fatty strap structue along the Stomach Meridians in rats, and another two points （A1, A2） beside st 36 were selected to be aeupunctured as control.The rats were killed by dissecting head after acupunctured, and the fatty strap structures were enucleated and homogenated. Radioimmunology assay was applied to determine the contents of β-endor- phin （β-EP）, Dynorphin A （DynA）, substance P （SP） in samples.Results： Unpaired t-test anylasis showed β-EP level was differently higher following st36 acupunctured than A1, A2 （ 19.08 ± 7.42 versus 2.27 ±1.99; t = 5.5374, P 〈 0.01 ）, DynA was significantly higher following st36 acupunctured than A1, A2 （23.08 ±8.61 versus 2.19 ±1.81; t =5.8194, P 〈 0.01）, but the difference betweent st36 and A1, A2 acupunctured did not reach the statistical significance （1.57 ± 0.49 versus 1.05 ±0.60; t = 1.6330, P 〉0.05） .Conclusions： Specifical analgesia effect substances β-EP and DynA increase in fatty strap structure along the Stomach Meridians following st 36 acupunctured in rats.

关 键 词：脂肪条带结构 后三里 胃经针刺镇痛 Β-EP DynA 

分 类 号：R245[医药卫生—针灸推拿学]