大鼠脑微血管内皮细胞条件培养液对皮层神经元活性的影响以及通络救脑注射液的干预作用  

Influence of Conditioned Medium of Rat Brain Microvascular Endothelial Cells on Activity of Cortical Neurons and Protective Effect of Tong Luo Jiu Nao Injection

作　　者：李卫红[1] 青雪梅[1] 华茜[1] 胡京红[1] 洪庆涛[1] 朱晓磊[1] 李澎涛[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《中华中医药杂志》2006年第6期334-337,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家"973"项目(2005CB523311);国家自然科学基金资助项目(30572284)

摘　　要：目的:探讨脑微血管内皮细胞条件培养液对神经元活性的影响,以及通络救脑注射液对此过程的干预作用。方法:制备正常、正常用药、拟缺血损伤、拟缺血损伤用药4种脑微血管内皮细胞条件培养液(N-CM、NT-CM、I-CM、IT-CM),分别作用于培养的皮层正常神经元和拟缺血损伤神经元,通过四甲基偶氮唑盐(MTT)比色法、乳酸脱氢酶(LDH)漏出率检测分析各个条件培养液对神经元活性的影响特征。结果:①N-CM对正常神经元无明显影响,但对拟缺血损伤的神经元表现出一定的保护作用,尤其是NT-CM可明显提高受损的神经元活性;②I-CM可降低正常神经元活性,对已受损的神经元则进一步加重损伤,IT-CM可明显改善这种损伤状态。结论:脑微血管内皮细胞的旁分泌作用可能在中枢系统缺血性损伤中起重要作用,有可能是不能透过血脑屏障的中药成分发挥治疗作用的靶点。Objective： To study the influence of conditioned medium of rat brain microvascular endothelial cells on the activity of cortical neurons and the protective effect of Tong Luo Jiu Nao Injection. Methods： We collected the conditioned media from 4 different cultured endothelial cell groups, which were normal endothelial cells, the normal ones and treated with Tong Luo Jiu Nao Injection, the injured ones damaged by simulated cerebral ischemia, as well as the injured ones and treated with Tong Luo Jiu Nao Injection, respectively （N-CM, NT-CM, I-CM, IT-CM）. Then, the conditioned medium was added into the cultures of the normal neurons and the damage ones which are injured by simulated cerebral ischemia as well, respectively. The effect of each type of conditioned medium on the activities of neurons was determined through the measurement of MTT and the tmnsduction rate of LDH. Results： （1） N-CM has no obvious effect on the normal neurons, but does show some protective effect on the injured ones by increasing its activity significantly; （2） I-CM could decrease the activity of the normal neurons as well as aggravate the damage on the injured ones, while this injury effect can be reversed remarkably by IT-CM. Conclusion： The paracrine secretion of the brain microvascular endothelial cells might be one of the vital mechanisms in cerebral ischemic injury, indicating that the brain microvascular endothelial cells could be the therapeutic targets of Chinese medicine, which are not able to permeate through the Blood-Brain Barrier.

关 键 词：脑微血管内皮细胞 条件培养液 皮层神经元 通络救脑注射液 

分 类 号：R285.5[医药卫生—中药学]