微卫星DNA监控大鼠近交系的培育  

Microsatellite DNA Monitoring in Inbred Cultivation of Outbred Rats

作　　者：李瑞生[1] 董罡[1] 吴晓燕[1] 王鹏[1] 王晓辉[2] 陈振文[2] 

机构地区：[1]军事医学科学院实验动物中心,北京100071 [2]首都医科大学实验动物科学部,北京100054

出　　处：《遗传》2006年第7期821-824,共4页Hereditas（Beijing)

基　　金：北京市自然科学基金课题资助(编号:5012012)~~

摘　　要：采用微卫星DNA技术来监控大鼠仔代基因状况，选择性地进行交配繁殖，使基因快速纯合，缩短培育新的近交系动物周期。利用PCR扩增30个微卫星DNA位点对封闭群SD和Wistar大鼠交配繁殖的仔代鼠进行微卫星DNA多态性分析，仔代中与母代SD大鼠相似系数高的与中的进行定向交配繁殖。F2代大鼠均为杂合多态的位点，没有纯合位点；到F9代时基因纯合位点达27个，纯合基因位点率为90％。每代相似系数具有不断上升的趋势，上升率为6％～20％。采用皮肤移植方法验证了F9代大鼠间无排斥反应。从而建立了一种新的快速培育近交系动物的方法。Microsatellite DNA was used to monitor the gene inheritance of successive generations of rats. It is useful in selective breeding to quickly bring genes to homozygosity and to shorten the cultivation periods of new inbreeding of rats. Thirty microsatellite DNA loci were PCR amplified to analyze the DNA polymorphism in generational crosses between the outbred Wistar and SD rats. Rats in F generations with a moderate to high coefficient of similarithy with the female F0 SD rats were crossed. All loci in the F2 generation were heterozygous and polymorphic. By F9, 27 loci have become homozygotic, with a homozygosity rate of 90%. The coefficient of similarity tended to increase at a rate of 6%～20% with each successive generation. Skin grafting confirmed no rejection among the F9 generation of rats. Therefore we have established a novel method for rapid cultivation of inbred rats.

关 键 词：微卫星DNA 大鼠 封闭群 近交系培育 

分 类 号：Q95-33[生物学—动物学]