温度及灌流液改性剂对青藤碱微透析探针体外物质转移系数的影响  

Influence of Temperature and Perfusate Modifier on In-vitro Mass Transfer Coefficient of Microdialysis with Sinomenine as a Model Drug

作　　者：张英丰[1] 周莉玲[2] 

机构地区：[1]广州中医药大学,2003级博士研究生广州510405 [2]广州中医药大学中药学院,广州510405

出　　处：《广州中医药大学学报》2006年第4期332-335,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:30371783);广东省自然科学基金资助项目(编号:31473);广州市科技计划项目(编号:2002Z3-E5011)

摘　　要：【目的】考察温度及灌流液改性剂对青藤碱微透析探针体外物质转移系数的影响。【方法】采用高效液相色谱仪分别测定不同温度下灌流液中乙醇的不同浓度对探针体外相对回收率的影响,并计算物质转移系数(k)。【结果】随着温度及乙醇比例的增加,相对回收率增加,物质转移系数亦随之增加。【结论】温度及乙醇比例可明显影响青藤碱微透析探针的相对回收率及物质转移系数。[ Objective ] To observe the influence of temperature and perfusate modifier on in-vitro mass transfer coefficient of microdialysis with sinomenine as a model drug. [Methods] The in-vitro relative recovery （RR） was assayed by HPLC at different temperatures and in perfusate including different concentrations of alcohol, and then the mass transfer coefficient was calculated. [Results] In-vitro RR increased with the temperature and the concentrations of alcohol, so did the mass transfer coefficient. [ Conclusion] Temperature and alcohol at different concentrations have an effect on RR and mass transfer coefficient of microdialysis with sinomenine as a model drug.

关 键 词：青藤碱/化学 微透析技术 色谱法 高效液相 

分 类 号：R284.1[医药卫生—中药学]