幽门螺旋杆菌感染者尿液中特异性IgG及亚类的检测  

Detection of IgG antibody and subclass in urine of patients infected with Helicobacter pylori

作　　者：吴永胜[1] 高世同[1] 黄达娜[1] 耿艺介[1] 张仁利[1] 

机构地区：[1]深圳市疾病预防控制中心,广东深圳518020

出　　处：《中国热带医学》2006年第7期1127-1128,共2页China Tropical Medicine

基　　金：国家教委留学回国人员科研启动基金(2001[345])

摘　　要：目的研究幽门螺旋杆菌感染者尿液中特异性循环抗体在幽门螺旋杆菌诊断上的应用。方法采集经C14尿素呼气实验确诊的幽门螺旋杆菌患者尿液,ELISA法测定尿液中抗幽门螺旋杆菌特异性IgG及亚类(IgG1,IgG2,IgG3)抗体;同时用健康人群尿液作为阴性对照。结果C14尿素呼气实验幽门螺旋杆菌患者尿液中抗幽门螺旋杆菌IgG抗体的检出率为96%(47/49),其中IgG1为96%(47/49),IgG2为20%(9/49),IgG3为32%(16/49),健康人群尿液中有4%的假阳性,但在健康人群中无幽门螺旋杆菌IgG2和IgG3特异性抗体检出。结论幽门螺旋杆菌患者尿液中存在抗幽门螺旋杆菌的特异性循环抗体,提示尿液中的抗体可以作为幽门螺旋杆菌感染的诊断标志,特别是尿液中的IgG1抗体可以作为幽门螺旋杆菌病的诊断的依据。Objective To explore the value of detection of IgG antibody in the urine of patients with Helicobacter pylori （HP） for diagnosis of Helicobacter pylori infection. Methods Urine samples were collected from patients with Helicobacter pylori, IgG antibody and its subclass（IgG1, IgG2, IgG3）were detected with ELISA, and normal urine samples were also conllected as negative control. Results Detection rate of specific IgG antibody in urine of patients with Helicobacter pylori was 96 % （47/49）, and the rates of IgG1, IgG2 and IgG3 were 96% （47/49）, 20% （9/49） and 32% （ 16/49, respectively. Although false positive results were observed in negative control group, but specific IgG2 and IgG3 antibody to Helicobacter pylori were not detected from those of nega- tive control. Conclusion Antibody to Helicobacter pylori exist in urine of patients with Helicobacter pylori and it can be taken as the basis for diagnosis of Helicobacter pylori infection.

关 键 词：幽门螺旋杆菌 尿液 IGG抗体 诊断 

分 类 号：R377[医药卫生—病原生物学]