乙肝HBV DNA荧光定量检测与血清标志物结果相关性分析  

Analysis of correlation of quantitative fluorescent detection of HBV DNA and the results of serum marker

作　　者：朱子犁[1] 古丽巴哈尔 刘建军[1] 阳帆[1] 汪洪富[1] 张海龙[1] 何建凡[1] 

机构地区：[1]深圳市疾病预防控制中心,广东深圳518020 [2]新疆哈密地区卫生防疫站,新疆哈密839000

出　　处：《中国热带医学》2006年第7期1138-1139,共2页China Tropical Medicine

摘　　要：目的研究乙肝血清学标志物(HBV-M)结果与HBV-DNA水平的关系,分析HBV DNA阳性率在各年龄组的分布情况。方法对深圳市部分服务行业人员血清应用荧光定量PCR检测和ELISA法检测并对结果进行分析。结果1 781例乙肝血清学标志物阳性血清中有1 102例HBV-DNA阳性。其中883例1.3.5阳性(俗称“大三阳”)血清中HBV-DNA的阳性率为96.15%;722例1.4.5阳性(俗称“小三阳”)血清中HBV-DNA的阳性率为24.38%;138例1.5阳性血清中HBV-DNA的阳性率为38.41%;22例1.3阳性血清中HBV-DNA的阳性率为90.91%;11例HBsAg阳性血清中HBV-DNA阳性率为36.36%。结论两种方法检测乙肝标志物有密切的相关性。荧光定量PCR是检测HBV-DNA含量较精确的方法,能正确反映乙肝病毒的复制水平和活跃程度;HBsAg和HBeAg与HBV-DNA含量有着明显的相关关系。Objective To analyze the relationship between the level of HBV DNA and the HBV - M. Methods Serum samples from 1 781 serviee workers were deteeted by ELISA and divided into nine groups： HBsAg（ ＋ ）/HBeAg（ ＋ ）/HBeAb（ ＋ ）, HBsAg（ ＋ ）/HBeAb（ ＋ ）/HBeAb（ ＋ ）, HBsAg（ ＋ ）/HBeAb（ ＋ ）, HBsAg（ ＋ ）/HBeAg（ ＋ ）, HBsAb/HBeAb（ ＋ ）, HBeAb（ ＋ ）/ HBeAb（ ＋ ）, HBsAg（ ＋ ）/HBeAb（ ＋ ）, HBsAb（ ＋ ） and HBsAg（ ＋ ）. HBV DNA eoneentration was determined by fluorescent quantitative PCR. Results The positive rate of HBV - DNA in the group of HBsAg（ ＋ ）/HBeAg（ ＋ ）/HBeAb（ ＋ ） was 61.88% （1 102/1 781）, the positive rate in the group of HBsAg（ ＋ ）/HBeAb（ ＋ ）/HBeAb（ ＋ ） was 96.15% , the positive rate in the group of HBsAg（ ＋ ）/HBeAb（ ＋ ）/HBeAb（ ＋ ） was 24.38%, the positive rate in the group of HBsAg（ ＋ ）/HBeAb（ ＋ ） was 38. 41%, 90.91% in. the group of HBsAg（ ＋ ）/HBeAg（ ＋ ）, 36.36% in the group of HBeAg（ ＋ ） and the positive rate of HBV - DNA was 0 % in the other groups. There signifieant differenees between the different hepatitis B sera positive modes and the result of HBV DNA. The HBV DNA level showed an obvious correlation with the HBeAg eontent in scra. Conclusion A elose eorrelation exists between these two methods in the detection of HBV markers. The FQ - PCR is a relatively aeurate method for detection of HBV - DNA.

关 键 词：乙肝病毒 HBV-DNA ELISA 荧光定量PCR 相关性 

分 类 号：R373.2[医药卫生—病原生物学]