运用流式细胞术对细胞内雄激素受体检测的研究  

Detection of androgen receptor in cells by flow cytometry

作　　者：梁春红[1] 李小鹰[1] 楚新梅[1] 

机构地区：[1]解放军总医院南楼心血管一科,北京100853

出　　处：《中华老年心脑血管病杂志》2006年第7期445-447,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的建立一种灵敏度高、特异性强的细胞内雄激素受体检测方法。探讨原发性高血压患者雄激素受体变化与原发性高血压的关系。方法运用流式细胞术检测112例男性高血压患者(高血压组)及120例健康男性体检者(对照组)雄激素受体的状态。结果流式细胞术不仅能定性而且能定量检测外周血白细胞的雄激素受体状态,且所需外周血量少。同时结合雄激素受体含量,来判断与原发性高血压的关系。结论流式细胞术检测雄激素受体更敏感、更具特异性,能更确切地指导临床医生的判断和治疗。Objectives cells and to investigate To establish a highly sensitive and specific method for detecting androgen receptor in the relationship between changes of androgen receptor and essential hypertension, Method Androgen receptor in 112 hypertensive men and 120 normotensive men was measured by means of flow eytometry. Results Flow cytometry analysis could measure the expression level of androgen receptor in peripheral blood leukocytes, which was more sensitive and needed less volume of blood compared with other methods. It also could be used to assess the relaxionship between androgen receptor content and essential hypertension. Conclusion The detection of androgen receptor in hypertension by flow cytometry is helpful for evaluating prognosis and selecting treatment.

关 键 词：高血压 流式细胞术 受体 雄激素 白细胞 

分 类 号：R544.1[医药卫生—心血管疾病]