人IL-6、TNF-α对登革病毒感染人树突状细胞的影响  

Effect of IL-6 and TNF-α on Dengue virus infection of human dendritic cells

作　　者：史毓杰[1] 江振友[1] 曾抗[2] 

机构地区：[1]暨南大学医学院微生物学与免疫学教研室,广东广州510632 [2]南方医科大学南方医院皮肤科,广东广州510515

出　　处：《细胞与分子免疫学杂志》2006年第4期469-471,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家高技术研究发展计划(863)项目(2003AA219060);广东省医学科研课题资助(A2005340)

摘　　要：目的:探讨IL-6和TNF-α对登革病毒感染人树突状细胞(DC)的影响。方法:从人外周血中常规分离单核细胞,以GM-CSF和IL-4诱导成DC并进行形态学特征、细胞表型和淋巴细胞刺激能力的鉴定。用不同浓度的IL-6、TNF-α作用于Ⅱ型登革病毒(DV2)感染的DCs,于感染后6、24、48、72、96h收集上清,采用甲基纤维素微量病毒空斑法检测病毒的滴度,以MTT比色法测定IL-6和TNF-α对DV感染DC及正常DC数量的变化。结果:中、低浓度的IL-6对DC中DV2的增殖均有增强作用;高、中浓度的TNF-α对DC中DV2的增殖具有抑制作用。IL-6和TNF-α对DV感染DC及正常DC数量无明显影响。结论:IL-6和TNF-α通过对DC的影响在DV2感染中具有重要作用。AIM： To study the effect of IL-6 and TNF-α on Dengue virus （DV） infection of human dendritic cells （DCs）. METHODS： Monocytes isolated from healthy human peripheral blood were incubated in medium with GM- CSF and IL-4 for 7 - 10 days, and then were collected and identified by transmission electron microscope, immunohistochemistry and lymphocytes stimulatory ability assay. DCs infected with DV type Ⅱ （ DV2 ） were incubated with IL-6, TNF-α at high, medium and low concentration. The supernatants were collected at 6 h, 24 h, 48 h, 72 h and 96 h. The virus titers were determined by plaque assay and the number of DCs at 48 h postinfection was mensurated by MTT. RESULTS： IL-6 at medium or low concentration increased DV2 replication in DCs, whereas TNF-α at high and medium concentration inhibited DV2 replication in DCs. Cytokines had no effect on the number of normal DCs. CONCLUSION： IL-6 and TNF-α may play an important role in the pathogenicity and immunity of Dengue virus.

关 键 词：登革病毒 人树突状细胞 细胞因子 感染 

分 类 号：R392[医药卫生—免疫学]