红景天抑制人脐静脉内皮细胞生长的初步研究  

Inhibition of rhodiola on the growth of EVC-304 cell line

作　　者：隋岫兰[1] 杨锋[2] 陈荣华[3] 噶布[1] 闫慧[2] 张世馥[2] 张景玲[4] 

机构地区：[1]西藏自治区第二人民医院,西藏拉萨850002 [2]中国医学科学院基础医学研究所,北京100005 [3]西藏自治区第一人民医院,西藏拉萨850000 [4]山东省千佛山医院,山东济南250014

出　　处：《细胞与分子免疫学杂志》2006年第4期524-525,共2页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30160029)

摘　　要：目的:利用体外培养人脐静脉内皮细胞,观察中药红景天对细胞生长的影响,初步探讨急、慢性高原病患者服用中药红景天防治高原病及改善症状等的作用机制。方法:培养人脐静脉内皮细胞EVC-304,设对照组与加药组,加药组分别加入不同浓度的红景天,培养3d后计数。加药组及对照组细胞用瑞氏染料染色并拍照。收集细胞以流式细胞术检测细胞周期。结果:对照组细胞形态正常,成梭形,排列紧密,分散均匀。加药组细胞数量明显减少,细胞皱缩,聚集成团,形态各异。流式细胞术检测显示加药组G1期细胞含量增多,S期细胞减少。结论:红景天具有抑制血管内皮细胞生长的作用,可能是通过抑制细胞的增殖来抑制内皮细胞生长。抑制血管内皮细胞生长对于阻止血管内膜增生,防止形成肺动脉高压,降低慢性高原病发病率具有实际应用意义。AIM： To observe the effect of rhodiola on human umbilical vein endothelial cell line EVC-304. METHODS： EVC-304 was cultured and divided into two groups： control group and rhodiola-treated group. Three days after treatment, cell survival rate-drug concertration curve was obtained by counting the survival cells, and cells in each group were stained by Wright＇s stain and observed under microscope. Cell cycle was determined by flow cytometry （FCM）. RESULTS： The survival cells in rhodiola-treated group was much less than those in control group. More cells in rhodiola-treated group stayed in G1 phase while less in S phase when compared with those in control group by FCM. CONCLUSION： Rhodiola can inhibite the growth of human endothelial cell line EVC-304, perhaps through inhibiting the proliferation of the cells. This may lay the foundation for the mechanism study and clinical application of rhodiola in prevention of pulmonary artery hypertension.

关 键 词：红景天 脐静脉内皮细胞 细胞培养 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]