不同损伤条件下CIDE-B的变化与神经元凋亡的关系  

Relationship between CIDE-B change and neuronal apoptosis under various injuring conditions

作　　者：孙红振[1] 王正国[2] 朱佩芳[2] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所全军战创伤中心骨创伤科,重庆400042 [2]第三军医大学附属大坪医院野战外科研究所第四研究室,重庆400042

出　　处：《中华创伤杂志》2006年第7期535-538,共4页Chinese Journal of Trauma

基　　金：国家重点基础研究发展规划资助项目(G1999054206)

摘　　要：目的通过对周围神经火器伤和切割伤CIDE-B基因及蛋白的表达检测,并与神经细胞的凋亡进行对比,探讨CIDE-B的变化与神经元凋亡的关系。方法113只大耳白兔随机分为火器伤组、切割伤组、正常对照组。在伤后不同时相点取材,以RT-PCR法检测CIDE-B mRNA表达水平,原位杂交检测CIDE-B mRNA的形态学分布,Western blot检测CIDE-B的蛋白水平。结果神经损伤后脊髓内CIDE-B mRNA表达增强。火器伤组增加的幅度较大,第1天开始增加,7d达到峰值,持续到2周,4周回落；切割伤组表达增加延迟,幅度也小。蛋白水平的变化与此相一致。正常对照组脊髓组织有少量的CIDE-B mRNA表达,分布于脊髓神经元细胞胞浆内。结论周围神经损伤后脊髓神经元CIDE-B在mRNA、蛋白水平的表达均增强,组织学分布在脊髓灰质内,火器性损伤改变更加明显。Objective To investigate the relationship between CIDE-B change and apoptosis by detecting expression of CIDE-B gene and protein under various injuring conditions （ firearm injury and incision injury） and comparing neuronal apoptosis. Methods A total of 108 New Zealand rabbits were randomized into firearm injury group, incision injury group and control group. Samples were harvested at different time points after injury for measuring CIDE-B mRNA expression level by RT-PCR, demonstrating morphological distribution of CIDE-B mRNA by in situ hybridization and determining CIDE-B protein level by Western blot. Results Following nerve injury, CIDE-B mRNA expression in spinal cord was enhanced markedly in the firearm injury group, increased from at day 1, reached peak at day 7, lasted for two weeks and declined after four weeks. CIDE-B mRNA expression was increased late, with small range in the incision injury group. The changes in CIDE-B protein level accorded with those in CIDE-B mRNA level. There was slight CIDE-B mRNA expression, distributing in spinal cord neuronal cytoplasma, in spinal cord tissues in the control group. Conclusions Following peripheral nerve injury, expressions of CIDE-B mRNA and protein are enhanced in spinal cord neurons and distributed in the grey matter of spinal cord. Such enhancement is more marked after firearm injury than incision injury.

关 键 词：周围神经创伤 枪击 凋亡 CIDE-B 

分 类 号：R651.3[医药卫生—外科学]